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Abstract：
OBJECTIVE: Cervical cancer screening by primary human papilloma virus detection and cytology is fraught with low specificity and variable sensitivity, respectively. Cytology-histology correlation remains modest. Biomarkers associated with early genetic events in cervical squamous carcinogenesis and detectable in cytology material are likely to be relevant. Human telomerase RNA component (hTERC) gene overexpression and aneuploidy are promising candidates in view of their reported early and consistent association with cervical squamous oncogenesis.
METHODS: We analysed hTERC gene expression and chromosome 7 ploidy by fluorescent in-situ hybridisation (FISH) in 50 women with cytological precursor squamous intraepithelial lesions and available histology outcomes. Results were expressed as percentages of cells showing ≥3 signals, mean signals/nucleus, and maximum amplitude across various cytology and histology categories. Proportions of positive cases were calculated from threshold values derived from 6 controls. Distribution of above indices with respect to ≥cervical intraepithelial neoplasia 2 (CIN2) was explored.
RESULTS: For both genetic aberrations, there was significant positive correlation (for all indices) between the proportion of positive cases and worsening cytological and histological outcomes (P < .05), with significant intergroup differences (P < .05). High-grade lesions (≥CIN2) had significantly higher results compared toCONCLUSIONS: HTERC gene amplification and chromosome 7 ploidy showed positive association with cervical squamous carcinogenesis and could be relevant in settings of discrepant cytology-histology correlation.
METHODS: We analysed hTERC gene expression and chromosome 7 ploidy by fluorescent in-situ hybridisation (FISH) in 50 women with cytological precursor squamous intraepithelial lesions and available histology outcomes. Results were expressed as percentages of cells showing ≥3 signals, mean signals/nucleus, and maximum amplitude across various cytology and histology categories. Proportions of positive cases were calculated from threshold values derived from 6 controls. Distribution of above indices with respect to ≥cervical intraepithelial neoplasia 2 (CIN2) was explored.
RESULTS: For both genetic aberrations, there was significant positive correlation (for all indices) between the proportion of positive cases and worsening cytological and histological outcomes (P < .05), with significant intergroup differences (P < .05). High-grade lesions (≥CIN2) had significantly higher results compared to
摘要：
目的：宫颈癌的原发性人乳头状瘤病毒检测和细胞学筛查的特异性和敏感性低，分别。细胞学-组织学相关性仍然适度。与宫颈鳞状细胞癌变早期遗传事件相关的生物标志物以及在细胞学材料中可检测到的生物标志物可能是相关的。人类端粒酶RNA成分（hTERC）基因过表达和非整倍性是有希望的候选者，因为它们与宫颈鳞状细胞癌的早期和一致相关。
方法：我们通过荧光原位杂交（FISH）分析了50例具有细胞学前体鳞状上皮内病变和可用组织学结局的女性的hTERC基因表达和7号染色体倍性。结果表示为显示≥3个信号的细胞百分比，平均信号/细胞核，以及各种细胞学和组织学类别的最大振幅。从来自6个对照的阈值计算阳性病例的比例。探讨了上述指标相对于≥宫颈上皮内瘤变2（CIN2）的分布。
结果：对于两种遗传畸变，阳性病例比例与恶化的细胞学和组织学结局之间存在显著正相关（所有指标）（P<0.05）。组间差异显著(P<0.05)。高级别病变（≥CIN2）的结果明显高于结论：HTERC基因扩增和7号染色体倍性与宫颈鳞癌的发生呈正相关，可能与细胞学-组织学相关性差异有关。
方法：我们通过荧光原位杂交（FISH）分析了50例具有细胞学前体鳞状上皮内病变和可用组织学结局的女性的hTERC基因表达和7号染色体倍性。结果表示为显示≥3个信号的细胞百分比，平均信号/细胞核，以及各种细胞学和组织学类别的最大振幅。从来自6个对照的阈值计算阳性病例的比例。探讨了上述指标相对于≥宫颈上皮内瘤变2（CIN2）的分布。
结果：对于两种遗传畸变，阳性病例比例与恶化的细胞学和组织学结局之间存在显著正相关（所有指标）（P<0.05）。组间差异显著(P<0.05)。高级别病变（≥CIN2）的结果明显高于
