METHODS: We analysed hTERC gene expression and chromosome 7 ploidy by fluorescent in-situ hybridisation (FISH) in 50 women with cytological precursor squamous intraepithelial lesions and available histology outcomes. Results were expressed as percentages of cells showing ≥3 signals, mean signals/nucleus, and maximum amplitude across various cytology and histology categories. Proportions of positive cases were calculated from threshold values derived from 6 controls. Distribution of above indices with respect to ≥cervical intraepithelial neoplasia 2 (CIN2) was explored.
RESULTS: For both genetic aberrations, there was significant positive correlation (for all indices) between the proportion of positive cases and worsening cytological and histological outcomes (P < .05), with significant intergroup differences (P < .05). High-grade lesions (≥CIN2) had significantly higher results compared to
方法:我们通过荧光原位杂交(FISH)分析了50例具有细胞学前体鳞状上皮内病变和可用组织学结局的女性的hTERC基因表达和7号染色体倍性。结果表示为显示≥3个信号的细胞百分比,平均信号/细胞核,以及各种细胞学和组织学类别的最大振幅。从来自6个对照的阈值计算阳性病例的比例。探讨了上述指标相对于≥宫颈上皮内瘤变2(CIN2)的分布。
结果:对于两种遗传畸变,阳性病例比例与恶化的细胞学和组织学结局之间存在显著正相关(所有指标)(P<0.05)。组间差异显著(P<0.05)。高级别病变(≥CIN2)的结果明显高于