PDF(Pubmed)
Abstract：
Research on lipid peroxidation in food degradation, oil and fat nutrition, and age-related diseases has gained significant international attention for the view of improvement of societal health and longevity. In order to promote basic studies on these topics, a chemiluminescence detection-high performance liquid chromatography instrument using a high-sensitivity single photon counter as a detector was developed. This instrument enabled us to selectively detect and quantify lipid hydroperoxides, a primary product of lipid peroxidation reactions, as hydroperoxide groups at the lipid class level. Furthermore, an analytical method using liquid chromatography-tandem mass spectrometry has been established to discriminate the position and stereoisomerization of hydroperoxide groups in lipid hydroperoxides. Using these two methods, the reaction mechanisms of lipid peroxidation in food and in the body have been confirmed.
摘要：
食品降解中脂质过氧化的研究,油脂营养，以及与年龄有关的疾病在改善社会健康和长寿的观点方面得到了国际上的广泛关注。为了促进这些主题的基础研究，研制了以高灵敏度单光子计数器为检测器的化学发光检测-高效液相色谱仪。该仪器使我们能够选择性地检测和量化脂质过氧化氢，脂质过氧化反应的主要产物，作为脂质类别水平的氢过氧化物基团。此外，已建立了一种使用液相色谱-串联质谱的分析方法，以区分脂质氢过氧化物中氢过氧化物基团的位置和立体异构化。使用这两种方法，已经证实了食物和体内脂质过氧化的反应机制。
