Abstract:
Engineered biomaterials provide unique functions to overcome the bottlenecks seen in biomedicine. Hence, a technique for rapid and routine tests of collagen is required, in which the test items commonly include molecular weight, crosslinking degree, purity, and sterilization induced structural change. Among them, the crosslinking degree mainly influences collagen properties. In this study, second harmonic generation (SHG) and coherent anti-Stokes Raman scattering (CARS) microscopy are used in combination to explore the collagen structure at molecular and macromolecular scales. These measured parameters are applied for the classification and quantification among the different collagen scaffolds, which were verified by other conventional methods. It is demonstrated that the crosslinking status can be analyzed from SHG images and presented as the coherency of collagen organization that is correlated with the mechanical properties. Also, the comparative analyses of SHG signal and relative CARS signal of amide III band at 1,240 cm−1 to δCH2 band at 1,450 cm−1 of these samples provide information regarding the variation of the molecular structure during a crosslinking process, thus serving as nonlinear optical signatures to indicate a successful crosslinking.
摘要:
工程生物材料提供了独特的功能来克服生物医学中的瓶颈。因此,需要一种快速和常规的胶原蛋白测试技术,其中测试项目通常包括分子量,交联度,纯度,和灭菌引起的结构变化。其中,交联程度主要影响胶原蛋白的性能。在这项研究中,二次谐波发生(SHG)和相干反斯托克斯拉曼散射(CARS)显微镜结合使用,以探索分子和大分子尺度的胶原蛋白结构。这些测量参数应用于不同胶原支架之间的分类和量化,通过其他常规方法进行了验证。已证明可以从SHG图像中分析交联状态,并表示为与机械性能相关的胶原蛋白组织的相干性。此外,这些样品的SHG信号和1,240cm-1的酰胺III带的相对CARS信号到1,450cm-1的δCH2带的比较分析提供了有关交联过程中分子结构变化的信息,因此充当非线性光学特征以指示成功的交联。