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Abstract：
Little information is currently available on the epidemiology of parasitic and commensal protist species in captive non-human primates (NHP) and their zoonotic potential. This study investigates the occurrence, molecular diversity, and potential transmission dynamics of parasitic and commensal protist species in a zoological garden in southern Spain. The prevalence and genotypes of the main enteric protist species were investigated in faecal samples from NHP (n = 51), zookeepers (n = 19) and free-living rats (n = 64) by molecular (PCR and sequencing) methods between 2018 and 2019. The presence of Leishmania spp. was also investigated in tissues from sympatric rats using PCR. Blastocystis sp. (45.1%), Entamoeba dispar (27.5%), Giardia duodenalis (21.6%), Balantioides coli (3.9%), and Enterocytozoon bieneusi (2.0%) (but not Troglodytella spp.) were detected in NHP. Giardia duodenalis (10.5%) and Blastocystis sp. (10.5%) were identified in zookeepers, while Cryptosporidium spp. (45.3%), G. duodenalis (14.1%), and Blastocystis sp. (6.25%) (but not Leishmania spp.) were detected in rats. Blastocystis ST1, ST3, and ST8 and G. duodenalis sub-assemblage AII were identified in NHP, and Blastocystis ST1 in zookeepers. Giardia duodenalis isolates failed to be genotyped in human samples. In rats, four Cryptosporidium (C. muris, C. ratti, and rat genotypes IV and V), one G. duodenalis (assemblage G), and three Blastocystis (ST4) genetic variants were detected. Our results indicate high exposure of NHP to zoonotic protist species. Zoonotic transmission of Blastocysts ST1 was highly suspected between captive NHP and zookeepers.
摘要：
目前，关于圈养的非人灵长类动物（NHP）中寄生虫和共生原生物种的流行病学及其人畜共患潜力的信息很少。这项研究调查了这种情况，分子多样性,以及西班牙南部动物园中寄生和共生原生物种的潜在传播动态。在NHP的粪便样本中调查了主要肠道原生生物物种的患病率和基因型（n=51），2018年至2019年，通过分子（PCR和测序）方法，动物园管理员（n=19）和自由生活大鼠（n=64）。利什曼原虫的存在。还使用PCR在同胞大鼠的组织中进行了研究。囊胚。（45.1%），Entamoebadispar(27.5%),十二指肠贾第鞭毛虫(21.6%),大肠杆菌Balantioides(3.9%)，和肠孢子虫（2.0％）（但不是刺猬属。)在NHP中检测到。十二指肠贾第鞭毛虫（10.5％）和囊胚。（10.5％）是在动物园管理员中确定的，而隐孢子虫属。（45.3%），G.十二指肠白质(14.1%)，和囊胚。（6.25％）（但不是利什曼原虫。)在大鼠中检测到。在NHP中鉴定出囊胚ST1、ST3和ST8以及十二指肠球藻的亚组AII，和动物园管理员的囊胚ST1。十二指肠贾第鞭毛虫分离株未能在人类样品中进行基因分型。在老鼠身上,四隐孢子虫(C.Muris,C.拉蒂,和大鼠基因型IV和V)，一个十二指肠G（组合G），并检测到三个囊胚（ST4）遗传变异。我们的结果表明，NHP对人畜共患原生物种的高度暴露。圈养的NHP和动物园管理员之间高度怀疑囊胚ST1的人畜共患传播。
