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Abstract：
Neuronal damage secondary to traumatic brain injury (TBI) is a rapidly evolving condition, which requires therapeutic decisions based on the timely identification of clinical deterioration. Changes in S100B biomarker levels are associated with TBI severity and patient outcome. The S100B quantification is often difficult since standard immunoassays are time-consuming, costly, and require extensive expertise. A zero-length cross-linking approach on a cysteamine self-assembled monolayer (SAM) was performed to immobilize anti-S100B monoclonal antibodies onto both planar (AuEs) and interdigitated (AuIDEs) gold electrodes via carbonyl-bond. Surface characterization was performed by atomic force microscopy (AFM) and specular-reflectance FTIR for each functionalization step. Biosensor response was studied using the change in charge-transfer resistance (Rct) from electrochemical impedance spectroscopy (EIS) in potassium ferrocyanide, with [S100B] ranging 10-1000 pg/mL. A single-frequency analysis for capacitances was also performed in AuIDEs. Full factorial designs were applied to assess biosensor sensitivity, specificity, and limit-of-detection (LOD). Higher Rct values were found with increased S100B concentration in both platforms. LODs were 18 pg/mL(AuES) and 6 pg/mL(AuIDEs). AuIDEs provide a simpler manufacturing protocol, with reduced fabrication time and possibly costs, simpler electrochemical response analysis, and could be used for single-frequency analysis for monitoring capacitance changes related to S100B levels.
摘要：
继发于创伤性脑损伤（TBI）的神经元损伤是一种快速发展的疾病，这需要基于及时识别临床恶化的治疗决策。S100B生物标志物水平的变化与TBI严重程度和患者预后相关。S100B定量通常很困难，因为标准的免疫测定是耗时的，昂贵的,需要广泛的专业知识。在半胱胺自组装单层（SAM）上进行零长度交联方法，以通过羰基键将抗S100B单克隆抗体固定在平面（AuEs）和叉指金（AuIDEs）电极上。通过原子力显微镜（AFM）和镜面反射FTIR对每个功能化步骤进行表面表征。使用亚铁氰化钾中电化学阻抗谱（EIS）的电荷转移电阻（Rct）的变化研究了生物传感器响应，[S100B]范围为10-1000pg/mL。还在AuIDE中进行了电容的单频分析。全阶乘设计用于评估生物传感器的灵敏度，特异性，和检测限（LOD）。在两个平台中,随着S100B浓度的增加,发现更高的Rct值。LOD为18pg/mL(AuES)和6pg/mL(AuIDE)。AuIDE提供了更简单的制造协议，减少了制造时间和可能的成本，更简单的电化学响应分析，可用于单频分析，以监测与S100B水平相关的电容变化。
