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Abstract：
Some Aeromonas species, potentially pathogenic for humans, are known to express up to three different classes of chromosomal β-lactamases, which may become hyperproduced and cause treatment failure. The aim of this study was to assess the utility of these species-specific β-lactamase genes as phylogenetic markers using whole-genome sequencing data. Core-genome alignments were generated for 36 Aeromonas genomes from seven different species and scanned for antimicrobial resistance genes. Core-genome alignment confirmed the MALDI-TOF identification of most of the isolates and re-identified an A. hydrophila isolate as A. dhakensis. Three (B, C and D) of the four Ambler classes of β-lactamase genes were found in A. sobria, A. allosacharophila, A. hydrophila and A. dhakensis (blaCphA, blaAmpC and blaOXA). A. veronii only showed class-B- and class-D-like matches (blaCphA and blaOXA), whereas those for A. media, A. rivipollensis and A. caviae were class C and D (blaCMY, blaMOX and blaOXA427). The phylogenetic tree derived from concatenated sequences of β-lactamase genes successfully clustered each species. Some isolates also had resistance to sulfonamides, quinolones and aminoglycosides. Whole-genome sequencing proved to be a useful method to identify Aeromonas at the species level, which led to the unexpected identification of A. dhakensis and A.rivipollensis and revealed the resistome of each isolate.
摘要：
一些气单胞菌物种，对人类有潜在的致病性，已知表达多达三种不同类型的染色体β-内酰胺酶，可能会过度生产并导致治疗失败。这项研究的目的是使用全基因组测序数据评估这些物种特异性β-内酰胺酶基因作为系统发育标记的实用性。对来自七个不同物种的36个气单胞菌基因组进行了核心基因组比对，并扫描了抗菌素抗性基因。核心基因组比对证实了大多数分离株的MALDI-TOF鉴定，并重新鉴定了嗜水A。三(B,在A.sobria中发现了4种Ambler类β-内酰胺酶基因的C和D），A.嗜酸性细菌，A.hydrophilaandA.dhakensis(blaCphA,blaAmpC和blaOXA）。A.veronii只显示B类和D类的比赛（blaCphA和blaOXA），而对于A.媒体来说，A.rivipollensis和A.caviae是C类和D类(blaCMY，blaMOXandblaOXA427).来自β-内酰胺酶基因的串联序列的系统发育树成功地聚集了每个物种。一些分离株对磺胺类药物也有抗性，喹诺酮类和氨基糖苷类。全基因组测序被证明是在物种水平上鉴定气单胞菌的有用方法，这导致了达克氏A和rivipollensis的意外鉴定，并揭示了每种分离株的抗性。
