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Abstract：
Growth factors execute essential biological functions and affect various physiological and pathological processes, including peripheral nerve repair and regeneration. Our previous sequencing data showed that the mRNA coding for betacellulin (Btc), an epidermal growth factor protein family member, was up-regulated in rat sciatic nerve segment after nerve injury, implying the potential involvement of Btc during peripheral nerve regeneration.
Expression of Btc was examined in Schwann cells by immunostaining. The function of Btc in regulating Schwann cells was investigated by transfecting cultured cells with siRNA segment against Btc or treating cells with Btc recombinant protein. The influence of Schwann cell-secreted Btc on neurons was determined using a co-culture assay. The in vivo effects of Btc on Schwann cell migration and axon elongation after rat sciatic nerve injury were further evaluated.
Immunostaining images and ELISA outcomes indicated that Btc was present in and secreted by Schwann cells. Transwell migration and wound healing observations showed that transfection with siRNA against Btc impeded Schwann cell migration while application of exogenous Btc advanced Schwann cell migration. Besides the regulating effect on Schwann cell phenotype, Btc secreted by Schwann cells influenced neuron behavior and increased neurite length. In vivo evidence supported the promoting role of Btc in nerve regeneration after both rat sciatic nerve crush injury and transection injury.
Our findings demonstrate the essential roles of Btc on Schwann cell migration and axon elongation and imply the potential application of Btc as a regenerative strategy for treating peripheral nerve injury.
Expression of Btc was examined in Schwann cells by immunostaining. The function of Btc in regulating Schwann cells was investigated by transfecting cultured cells with siRNA segment against Btc or treating cells with Btc recombinant protein. The influence of Schwann cell-secreted Btc on neurons was determined using a co-culture assay. The in vivo effects of Btc on Schwann cell migration and axon elongation after rat sciatic nerve injury were further evaluated.
Immunostaining images and ELISA outcomes indicated that Btc was present in and secreted by Schwann cells. Transwell migration and wound healing observations showed that transfection with siRNA against Btc impeded Schwann cell migration while application of exogenous Btc advanced Schwann cell migration. Besides the regulating effect on Schwann cell phenotype, Btc secreted by Schwann cells influenced neuron behavior and increased neurite length. In vivo evidence supported the promoting role of Btc in nerve regeneration after both rat sciatic nerve crush injury and transection injury.
Our findings demonstrate the essential roles of Btc on Schwann cell migration and axon elongation and imply the potential application of Btc as a regenerative strategy for treating peripheral nerve injury.
摘要：
生长因子执行基本的生物学功能，影响各种生理和病理过程，包括周围神经修复和再生。我们之前的测序数据显示，编码β细胞蛋白（Btc）的mRNA，表皮生长因子蛋白家族成员，神经损伤后大鼠坐骨神经节段上调，暗示Btc可能参与周围神经再生。
通过免疫染色在施旺细胞中检测Btc的表达。通过用针对Btc的siRNA片段转染培养的细胞或用Btc重组蛋白处理细胞，研究了Btc在调节雪旺细胞中的功能。使用共培养测定法测定施万细胞分泌的Btc对神经元的影响。进一步评估了Btc对大鼠坐骨神经损伤后雪旺细胞迁移和轴突伸长的体内影响。
免疫染色图像和ELISA结果表明Btc存在于雪旺氏细胞中并由其分泌。Transwell迁移和伤口愈合观察表明，用针对Btc的siRNA转染阻碍了雪旺细胞的迁移，而外源Btc的应用促进了雪旺细胞的迁移。除了对雪旺细胞表型的调节作用外，雪旺氏细胞分泌的Btc影响神经元行为并增加神经突长度。体内证据支持Btc在大鼠坐骨神经挤压伤和横断伤后的神经再生中的促进作用。
我们的发现证明了Btc对雪旺细胞迁移和轴突伸长的重要作用，并暗示了Btc作为治疗周围神经损伤的再生策略的潜在应用。
通过免疫染色在施旺细胞中检测Btc的表达。通过用针对Btc的siRNA片段转染培养的细胞或用Btc重组蛋白处理细胞，研究了Btc在调节雪旺细胞中的功能。使用共培养测定法测定施万细胞分泌的Btc对神经元的影响。进一步评估了Btc对大鼠坐骨神经损伤后雪旺细胞迁移和轴突伸长的体内影响。
免疫染色图像和ELISA结果表明Btc存在于雪旺氏细胞中并由其分泌。Transwell迁移和伤口愈合观察表明，用针对Btc的siRNA转染阻碍了雪旺细胞的迁移，而外源Btc的应用促进了雪旺细胞的迁移。除了对雪旺细胞表型的调节作用外，雪旺氏细胞分泌的Btc影响神经元行为并增加神经突长度。体内证据支持Btc在大鼠坐骨神经挤压伤和横断伤后的神经再生中的促进作用。
我们的发现证明了Btc对雪旺细胞迁移和轴突伸长的重要作用，并暗示了Btc作为治疗周围神经损伤的再生策略的潜在应用。
