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Abstract：
BACKGROUND: The intracavernosal (IC) injection of chitosan activated platelet rich plasma (cPRP) has shown to improve the erectile dysfunction in cavernous nerve injury rat model. However, the action target of PRP in improving neurogenic erectile dysfunction remains unclear. We aimed to determine the effect of cPRP action at early stage that further mediates its effect on erectile function (EF) recovery in the bilateral cavernous nerve crushing (BCNC) injury rat model.
METHODS: Fifty-four rats were randomly divided into two equal groups: intracavernosal ( IC) injection of saline after BCNC (group 1) and IC injection of cPRP after BCNC (group 2). Five animals in each group were euthanized at 3, 7 and 14 day (d) post-injection, and the tissues were harvested to conduct transmission electron microscopy and histological assays. Six animals in each group were used to determine the recovery of EF at 14 and 28 d post-injury.
RESULTS: IC injections of cPRP increased all EF parameters at 28 d and 14 d post-injury (p < 0.05). cPRP injections simultaneously prevented the loss of neuronal nitric oxide synthase-positive neurons (p < 0.05) and nerve fibers (p < 0.05) in the major pelvic ganglion and cavernous nerve (CN), respectively, compared with saline injections. This simultaneous accelerated the regeneration of myelinated axons of the CN, reduced apoptosis, and enhanced the proliferation of the corporal smooth muscle cells at an earlier stage.
CONCLUSIONS: These results suggest that the application of cPRP was beneficial to restore EF via neuroprotective and tissue-protective effects at early stage.
METHODS: Fifty-four rats were randomly divided into two equal groups: intracavernosal ( IC) injection of saline after BCNC (group 1) and IC injection of cPRP after BCNC (group 2). Five animals in each group were euthanized at 3, 7 and 14 day (d) post-injection, and the tissues were harvested to conduct transmission electron microscopy and histological assays. Six animals in each group were used to determine the recovery of EF at 14 and 28 d post-injury.
RESULTS: IC injections of cPRP increased all EF parameters at 28 d and 14 d post-injury (p < 0.05). cPRP injections simultaneously prevented the loss of neuronal nitric oxide synthase-positive neurons (p < 0.05) and nerve fibers (p < 0.05) in the major pelvic ganglion and cavernous nerve (CN), respectively, compared with saline injections. This simultaneous accelerated the regeneration of myelinated axons of the CN, reduced apoptosis, and enhanced the proliferation of the corporal smooth muscle cells at an earlier stage.
CONCLUSIONS: These results suggest that the application of cPRP was beneficial to restore EF via neuroprotective and tissue-protective effects at early stage.
摘要：
背景：在海绵体神经损伤大鼠模型中，壳聚糖激活的富血小板血浆（cPRP）的海绵体内注射（IC）可改善勃起功能障碍。然而,PRP在改善神经源性勃起功能障碍方面的作用目标尚不清楚.我们旨在确定早期cPRP作用的作用，该作用进一步介导其对双侧海绵体神经挤压（BCNC）损伤大鼠模型中勃起功能（EF）恢复的影响。
方法：54只大鼠随机分为两组：BCNC后海绵体内（IC）注射生理盐水（组1）和BCNC后IC注射cPRP（组2）。每组五只动物在注射后第3、7和14天（d）安乐死，采集组织进行透射电镜和组织学检测。每组6只动物用于确定损伤后14和28d的EF恢复。
结果：IC注射cPRP增加了伤后28d和14d的所有EF参数（p<0.05）。cPRP注射同时防止神经元一氧化氮合酶阳性神经元的损失（p<0.05）和神经纤维（p<0.05）在主要的骨盆神经节和海绵体神经（CN），分别,与生理盐水注射相比。这同时加速了CN的有髓轴突的再生，减少细胞凋亡，并在早期增强了身体平滑肌细胞的增殖。
结论：这些结果表明，cPRP的应用有利于早期通过神经保护和组织保护作用恢复EF。
方法：54只大鼠随机分为两组：BCNC后海绵体内（IC）注射生理盐水（组1）和BCNC后IC注射cPRP（组2）。每组五只动物在注射后第3、7和14天（d）安乐死，采集组织进行透射电镜和组织学检测。每组6只动物用于确定损伤后14和28d的EF恢复。
结果：IC注射cPRP增加了伤后28d和14d的所有EF参数（p<0.05）。cPRP注射同时防止神经元一氧化氮合酶阳性神经元的损失（p<0.05）和神经纤维（p<0.05）在主要的骨盆神经节和海绵体神经（CN），分别,与生理盐水注射相比。这同时加速了CN的有髓轴突的再生，减少细胞凋亡，并在早期增强了身体平滑肌细胞的增殖。
结论：这些结果表明，cPRP的应用有利于早期通过神经保护和组织保护作用恢复EF。
