PDF(Sci-hub)
Abstract：
BACKGROUND: Lactobacillus spp. have been researched worldwide and are used in probiotics, but due to difficulties with laboratory cultivation of and experimentation on oral microorganisms, there are few reports of Lactobacillus spp. being isolated from the oral cavity and tested against oral pathogens. This research sought to isolate and determine the safety and inhibitory capabilities of a Lactobacillus culture taken from the human body.
RESULTS: One organism was isolated, named \"L. gasseri HHuMIN D\", and evaluated for safety. A 5% dilution of L. gasseri HHuMIN D culture supernatant exhibited 88.8% inhibition against halitosis-producing anaerobic microorganisms and the organism itself exhibited powerful inhibitory effects on the growth of 11 oral bacteria. Hydrogen peroxide production reached 802 μmol/L after 12 h and gradually diminished until 24 h, it efficiently aggregated with P. catoniae and S. sanguinis, and it completely suppressed S. mutans-manufactured artificial dental plaque. L. gasseri HHuMIN D\'s KB cell adhesion capacity was 4.41 cells per cell, and the cell adhesion of F. nucleatum and S. mutans diminished strongly in protection and displacement assays.
CONCLUSIONS: These results suggest that L. gasseri HHuMIN D is a safe, bioactive, lactobacterial food ingredient, starter culture, and/or probiotic microorganism for human oral health.
RESULTS: One organism was isolated, named \"L. gasseri HHuMIN D\", and evaluated for safety. A 5% dilution of L. gasseri HHuMIN D culture supernatant exhibited 88.8% inhibition against halitosis-producing anaerobic microorganisms and the organism itself exhibited powerful inhibitory effects on the growth of 11 oral bacteria. Hydrogen peroxide production reached 802 μmol/L after 12 h and gradually diminished until 24 h, it efficiently aggregated with P. catoniae and S. sanguinis, and it completely suppressed S. mutans-manufactured artificial dental plaque. L. gasseri HHuMIN D\'s KB cell adhesion capacity was 4.41 cells per cell, and the cell adhesion of F. nucleatum and S. mutans diminished strongly in protection and displacement assays.
CONCLUSIONS: These results suggest that L. gasseri HHuMIN D is a safe, bioactive, lactobacterial food ingredient, starter culture, and/or probiotic microorganism for human oral health.
摘要：
背景：乳杆菌属。已经在世界范围内进行了研究，并用于益生菌，但是由于口腔微生物的实验室培养和实验困难，关于乳杆菌属的报道很少。从口腔中分离并针对口腔病原体进行测试。这项研究试图分离并确定取自人体的乳杆菌培养物的安全性和抑制能力。
结果：分离出一种生物，名为\"L.GasseriHHuMIND\“，并进行安全性评估。5％稀释的L.gasseriHHuMIND培养上清液对产生口臭的厌氧微生物具有88.8％的抑制作用，并且该生物本身对11种口腔细菌的生长具有强大的抑制作用。12h后过氧化氢产量达到802μmol/L，并逐渐减少至24h，它有效地聚集了卡氏杆菌和血链球菌，它完全抑制了变形链球菌制造的人造牙菌斑。L.gasseriHHuMIND的KB细胞粘附能力为4.41个细胞/细胞，在保护和置换试验中，核仁弧菌和变形链球菌的细胞粘附力强烈减弱。
结论：这些结果表明L.gasseriHHuMIND是一种安全的，生物活性，乳酸菌食品成分，starterculture,和/或用于人类口腔健康的益生菌微生物。
结果：分离出一种生物，名为\"L.GasseriHHuMIND\“，并进行安全性评估。5％稀释的L.gasseriHHuMIND培养上清液对产生口臭的厌氧微生物具有88.8％的抑制作用，并且该生物本身对11种口腔细菌的生长具有强大的抑制作用。12h后过氧化氢产量达到802μmol/L，并逐渐减少至24h，它有效地聚集了卡氏杆菌和血链球菌，它完全抑制了变形链球菌制造的人造牙菌斑。L.gasseriHHuMIND的KB细胞粘附能力为4.41个细胞/细胞，在保护和置换试验中，核仁弧菌和变形链球菌的细胞粘附力强烈减弱。
结论：这些结果表明L.gasseriHHuMIND是一种安全的，生物活性，乳酸菌食品成分，starterculture,和/或用于人类口腔健康的益生菌微生物。
