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Abstract：
UNASSIGNED: Biliary strictures after bile duct injury or duct-to-duct biliary reconstruction are serious complications that markedly reduce patients\' quality of life because their treatment involves periodic stent replacements. This study aimed to create a scaffold-free tubular construct as an interposition graft to treat biliary complications.
UNASSIGNED: Scaffold-free tubular constructs of allogeneic pig fibroblasts, that is, fibroblast tubes, were created using a Bio-3D Printer and implanted into pigs as interposition grafts for duct-to-duct biliary reconstruction.
UNASSIGNED: Although the fibroblast tube was weaker than the native bile duct, it was sufficiently strong to enable suturing. The pigs\' serum hepatobiliary enzyme levels remained stable during the experimental period. Micro-computed tomography showed no biliary strictures, no biliary leakages, and no intrahepatic bile duct dilations. The tubular structure was retained in all resected specimens, and the fibroblasts persisted at the graft sites. Immunohistochemical analyses revealed angiogenesis in the fibroblast tube and absence of extensions of the biliary epithelium into the fibroblast tube\'s lumen.
UNASSIGNED: This study\'s findings demonstrated successful reconstruction of the extrahepatic bile duct with a scaffold-free tubular construct created from pig fibroblasts using a novel Bio-3D Printer. This construct could provide a novel regenerative treatment for patients with hepatobiliary diseases.
UNASSIGNED: Scaffold-free tubular constructs of allogeneic pig fibroblasts, that is, fibroblast tubes, were created using a Bio-3D Printer and implanted into pigs as interposition grafts for duct-to-duct biliary reconstruction.
UNASSIGNED: Although the fibroblast tube was weaker than the native bile duct, it was sufficiently strong to enable suturing. The pigs\' serum hepatobiliary enzyme levels remained stable during the experimental period. Micro-computed tomography showed no biliary strictures, no biliary leakages, and no intrahepatic bile duct dilations. The tubular structure was retained in all resected specimens, and the fibroblasts persisted at the graft sites. Immunohistochemical analyses revealed angiogenesis in the fibroblast tube and absence of extensions of the biliary epithelium into the fibroblast tube\'s lumen.
UNASSIGNED: This study\'s findings demonstrated successful reconstruction of the extrahepatic bile duct with a scaffold-free tubular construct created from pig fibroblasts using a novel Bio-3D Printer. This construct could provide a novel regenerative treatment for patients with hepatobiliary diseases.
摘要：
■胆管损伤或胆管-胆管重建后的胆道狭窄是严重的并发症，显著降低患者的生活质量，因为他们的治疗包括定期更换支架。这项研究旨在创建一种无支架的管状结构，作为治疗胆道并发症的介入移植物。
■同种异体猪成纤维细胞的无支架管状结构，也就是说,成纤维细胞管,使用Bio-3D打印机创建并植入猪作为导管到导管胆道重建的插入移植物。
■虽然成纤维细胞管弱于天然胆管，它足够坚固以进行缝合。实验期间猪血清肝胆酶水平保持稳定。显微计算机断层扫描显示无胆道狭窄,没有胆漏,肝内胆管没有扩张.管状结构保留在所有切除的标本中，成纤维细胞持续存在于移植部位。免疫组织化学分析显示成纤维细胞管中的血管生成，并且胆管上皮没有延伸到成纤维细胞管腔中。
■这项研究的发现证明了使用新型Bio-3D打印机从猪成纤维细胞产生的无支架管状结构成功重建肝外胆管。该构建体可以为肝胆疾病患者提供新的再生治疗。
■同种异体猪成纤维细胞的无支架管状结构，也就是说,成纤维细胞管,使用Bio-3D打印机创建并植入猪作为导管到导管胆道重建的插入移植物。
■虽然成纤维细胞管弱于天然胆管，它足够坚固以进行缝合。实验期间猪血清肝胆酶水平保持稳定。显微计算机断层扫描显示无胆道狭窄,没有胆漏,肝内胆管没有扩张.管状结构保留在所有切除的标本中，成纤维细胞持续存在于移植部位。免疫组织化学分析显示成纤维细胞管中的血管生成，并且胆管上皮没有延伸到成纤维细胞管腔中。
■这项研究的发现证明了使用新型Bio-3D打印机从猪成纤维细胞产生的无支架管状结构成功重建肝外胆管。该构建体可以为肝胆疾病患者提供新的再生治疗。
