Electrochemical sensor for the assessment of carbohydrate deficient transferrin: Application to diagnosis of congenital disorders of glycosilation.

• 影响指数: 12.545
• 发表时间: May 2021 1
• 来源期刊: Biosens Bioelectron
• DOI: 10.1016/j.bios.2021.113098
• PMID: 33636501
• 文章类型: Journal Article
• 作者列表: Sierra T,Crevillen AG,Escarpa A

关键词: Carbohydrate deficient transferrin; Congenital disorders of glycosylation; Electrochemical tag; Rare disease; Screen printed carbon electrode

Mesh: Biomarkers; Biosensing Techniques; Glycoproteins; Glycosylation; Humans; Osmium; Transferrin

Abstract

Carbohydrate deficient transferrin (CDT) is used as biomarker of different health problems as, for example, congenital disorders of glycosylation (CDG). We propose a screen-printed-based electrochemical sensor for the determination of carbohydrate deficient transferrin using an Os (VI) tag-based electrochemistry. When transferrin is labeled with Os (VI) complex, it generates two voltammetric signals: one from carbohydrates (electrochemical signal of osmium (VI) complex at -0.9 V/Ag) and one from the amino acids present in glycoprotein (intrinsic electrochemical signal of glycoprotein at +0.8 V/Ag). The relationship between the two analytical signals (carbohydrate signal/protein signal) is an indicator of the degree of glycosylation (electrochemical index of glycosylation), which has shown an excellent correlation (r = 0.990) with the official parameter %CDT obtained by CE-UV. The suitability of this approach was demonstrated by analyzing serum samples from CDG patients.