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Abstract：
This study investigated the performance and microbiome of cyclic denitrification filters (CDFs) for wood and sulfur heterotrophic-autotrophic denitrification (WSHAD) of saline wastewater. Wood-sulfur CDFs integrated into two pilot-scale marine recirculating aquaculture systems achieved high denitrification rates (103 ± 8.5 g N/(m3·d)). The combined use of pine wood and sulfur resulted in lower SO42- accumulation compared with prior saline wastewater denitrification studies with sulfur alone. Although fish tank water quality parameters, including ammonia, nitrite, nitrate and sulfide, were below the inhibitory levels for marine fish production, lower survival rates of Poecilia sphenops were observed compared with prior studies. Heterotrophic denitrification was the dominant removal mechanism during the early operational stages, while sulfur autotrophic denitrification increased as readily biodegradable organic carbon released from wood chips decreased over time. 16S rRNA-based analysis of the CDF microbiome revealed that Sulfurimonas, Thioalbus, Defluviimonas, and Ornatilinea as notable genera that contributed to denitrification performance.
摘要：
这项研究调查了循环反硝化过滤器（CDF）对含盐废水的木材和硫异养-自养反硝化（WSHAD）的性能和微生物组。木材硫CDF整合到两个中试规模的海洋循环水产养殖系统中，实现了较高的反硝化速率（103±8.5gN/（m3·d））。与先前仅使用硫的含盐废水反硝化研究相比，松木和硫的结合使用导致SO42-积累更低。虽然鱼缸水质参数,包括氨,亚硝酸盐,硝酸盐和硫化物，低于海洋鱼类生产的抑制水平，与先前的研究相比，观察到的PoeciliaSpenops的存活率较低。在早期运行阶段，异养反硝化是主要的去除机制，而硫自养反硝化增加，因为木片释放的易于生物降解的有机碳随着时间的推移而减少。基于16SrRNA的CDF微生物组分析显示，Thioalbus,去氟单胞菌,和Ornatilinea是促成反硝化性能的著名属。
