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Abstract：
Electrospun fiber scaffolds, due to their mimicry of bone extracellular matrix (ECM), have become an important biomaterial widely applied in bone tissue engineering in recent years. While topographic cues of electrospun membranes such as alignment and diameter played vital roles in determining cellular behaviors. Yet few researches about the effects of these two significant parameters on osteogenesis have been reported. Thus, the present work explored the influence of aligned and random poly (L-lactic acid) (PLLA) fiber matrices with diameters of nanoscale (0.6 μm) and microscale (1.2 μm), respectively, on cellular responses of bone marrow mesenchymal stem cells (BMSCs), such as cell adhesion, migration, proliferation and osteogenesis. Our results revealed that aligned nanofibers (AN) could affect cell morphology and promote the migration of BMSCs after 24 h of cell culturing. Besides, AN group was observed to possess excellent biocompatibility and have significantly improved cell growth comparing with random nanofibers. More importantly, in vitro osteogenesis researches including ALP and Alizarin Red S staining, qRT-PCR and immunofluorescence staining demonstrated that BMSCs culturing on AN group exhibited higher osteogenic induction proficiency than that on aligned microfibers (AM) and random fiber substrates (RN and RM). Accordingly, aligned nanofiber scaffolds have greater application potential in bone tissue engineering.
摘要：
静电纺纤维支架，由于它们对骨胞外基质（ECM）的模仿，已成为近年来广泛应用于骨组织工程的重要生物材料。虽然电纺膜的形貌线索，如排列和直径在确定细胞行为中起着至关重要的作用。然而，关于这两个重要参数对成骨作用的研究很少。因此，本工作探索了排列和随机聚（L-乳酸）（PLLA）纤维基质的纳米级（0.6μm）和微米级（1.2μm）直径的影响，分别,骨髓间充质干细胞（BMSCs）的细胞反应，如细胞粘附，迁移,增殖和成骨。我们的结果表明，排列的纳米纤维（AN）可以影响细胞形态并促进BMSCs在细胞培养24h后的迁移。此外,与随机纳米纤维相比，AN组具有优异的生物相容性，细胞生长显着改善。更重要的是,体外成骨研究包括ALP和茜素红S染色,qRT-PCR和免疫荧光染色表明，在AN组中培养的BMSCs显示出比在对齐的微纤维（AM）和随机纤维基底（RN和RM）上更高的成骨诱导能力。因此，取向纳米纤维支架在骨组织工程中具有较大的应用潜力。
