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Abstract：
Background: The factor behind the activation of the remnant odontogenic tissues and development of odontogenic cysts and tumors is poorly understood.This study aimed to investigate the presence of human cytomegalovirus (HCMV) in dentigerous cyst (DC), odontogenic keratocyst (OKC), and ameloblastoma (AB). Methods: The study included 41 samples, which distributed into DC (n=13), OKC (n=12), and AB (n=16). Conventional PCR assay and IHC analysis were used to detect the HCMV-DNA and HCMV glycoprotein B (HCMV-gB) respectively. Results: HCMV-DNA was detected in 10 samples (62.5%) of AB, four samples (30.8%) of DC, and three samples (25 %) of OKC respectively (χ2 test = 1.195, p= 0.247). Meanwhile, HCMV-gB was found in 12 (75%) of AB, in 2 (15.4%) of DC, and absent in OKC (0.0%) (χ2 test = 4.122, p= 0.042). Conclusions: The high prevalence of HCMV inside the odontogenic epithelium of AB could indicate a possible role of the virus in the oncogenesis and/or oncomodulation of the AB. Additionally, we recommend the IHC for the detection of HCMV in the odontogenic tumors like AB.
摘要：
背景：对残余牙源性组织的激活以及牙源性囊肿和肿瘤的发展背后的因素知之甚少。本研究旨在探讨人巨细胞病毒（HCMV）在牙质囊肿（DC）中的存在，牙源性角化囊肿（OKC），成釉细胞瘤（AB）。方法：本研究包括41个样本，分布到DC(n=13)，OKC（n=12），和AB（n=16）。常规PCR和IHC分析分别用于检测HCMV-DNA和HCMV糖蛋白B(HCMV-gB)。结果:在10份(62.5%)AB标本中检测到HCMV-DNA,四个DC样本(30.8%)，和三个OKC样本（25％）（χ2检验=1.195，p=0.247）。同时,在12例(75%)AB中发现HCMV-gB，在DC的2（15.4%）中，OKC中不存在（0.0％）（χ2检验=4.122，p=0.042）。结论：AB牙源性上皮内HCMV的高患病率可能表明该病毒在AB的肿瘤发生和/或调控中可能发挥作用。此外,我们推荐IHC检测牙源性肿瘤如AB中的HCMV.
