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Abstract：
Enterocytozoon bieneusi can cause severe diarrhea in children and adults. However, in China, there are scant studies on E. bieneusi in diarrheal children and adults, with the exception of prevalence and genotyping data in a small number of cities including Hubei, Shanghai, and Heilongjiang. In this study, 196 fecal samples (n = 132 in Chongqing, n = 44 in Shandong, n = 20 in Hubei) were collected, including 91 from children and 105 from adults. Through microscopic examination, 19 positive samples (11 from children and 8 from adults) were detected. Using PCR examination, the internal transcriptional spacer (ITS) region was utilized by nested PCR to detect and characterize E. bieneusi. Twenty positive samples were detected, including 14 from children (≤11 years of age) and 6 from adults. According to the sequence analysis of ITS data, one known zoonotic (D) and seven novel (CQH5-11) genotypes were identified. This is the first molecular epidemiological study of E. bieneusi in diarrheal patients in different regions of China. Therefore, this study can provide useful information for the molecular epidemiology and control of E. bieneusi infection in humans in the future.
摘要：
肠孢子虫可导致儿童和成人严重腹泻。然而,在中国,关于腹泻儿童和成人的E.bieneusi的研究很少，除了包括湖北在内的少数城市的患病率和基因分型数据外，上海,和黑龙江。在这项研究中,196份粪便样本(重庆市n=132，山东n=44，湖北n=20)收集，其中儿童91人，成人105人。通过显微镜检查,检测到19个阳性样本（11个来自儿童，8个来自成人）。使用PCR检查，通过巢式PCR利用内部转录间隔区（ITS）区域来检测和表征E.bieneusi。检测到20个阳性样本，包括14名儿童（≤11岁）和6名成人。根据ITS数据的序列分析,确定了一种已知的人畜共患（D）和七个新的（CQH5-11）基因型。这是首次在中国不同地区腹泻患者中进行的E.bieneusi分子流行病学研究。因此,本研究可为今后人类E.bieneusi感染的分子流行病学和控制提供有用的信息。
