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Abstract：
Telangiectatic osteosarcoma (TOS) is an aggressive variant of osteosarcoma (OS) with distinctive radiographic, gross, microscopic features, and prognostic implications. Despite several studies on OS, we are still far from understanding the molecular mechanisms of TOS. In recent years, many studies have demonstrated not only that microRNAs (miRNAs) are involved in OS tumorigenesis, development, and metastasis, but also that the presence in high-grade types of OS of cancer stem cells (CSCs) plays an important role in tumor progression. Despite these findings, nothing has been described previously about the expression of miRNAs and the presence of CSCs in human TOS. Therefore, we have isolated/characterized a putative CSC cell line from human TOS (TOS-CSCs) and evaluated the expression levels of several miRNAs in TOS-CSCs using real-time quantitative assays. We show, for the first time, the existence of CSCs in human TOS, highlighting the in vitro establishment of this unique stabilized cell line and an identification of a preliminary expression of the miRNA profile, characteristic of TOS-CSCs. These findings represent an important step in the study of the biology of one of the most aggressive variants of OS and the role of miRNAs in TOS-CSC behavior.
摘要：
毛细血管扩张骨肉瘤（TOS）是骨肉瘤（OS）的一种侵袭性变体，具有独特的放射学特征，gross,微观特征，和预后影响。尽管对OS进行了一些研究，我们还远未了解TOS的分子机制。近年来,许多研究表明,microRNAs(miRNAs)不仅参与OS肿瘤发生,发展,和转移，而且，在高级别类型的OS中，癌症干细胞（CSC）的存在在肿瘤进展中起着重要作用。尽管有这些发现,先前没有描述关于人TOS中miRNA的表达和CSC的存在。因此,我们已经从人类TOS（TOS-CSC）中分离/表征了推定的CSC细胞系，并使用实时定量分析评估了TOS-CSC中几种miRNA的表达水平。我们展示,第一次,CSC在人类TOS中的存在，强调了这种独特的稳定细胞系的体外建立和miRNA谱的初步表达的鉴定，TOS-CSCs的特征。这些发现代表了OS最具侵略性变体之一的生物学研究以及miRNA在TOS-CSC行为中的作用的重要一步。
