关键词: De novo assembly GENEIOUS software Southern Hemisphere buckwheat family living collections substitution rates

来  源:   DOI:10.1080/23802359.2018.1456979   PDF(Sci-hub)   PDF(Pubmed)

Abstract:
We assembled the plastome of the temperate, Southern Hemisphere liana Muehlenbeckia australis from high throughput sequencing data (paired-end Illumina reads) generated from total genomic DNA sequencing libraries. M. australis\' chloroplast genome sequence (GenBank: MG604297) is 163,484 bp in length and composed of long single copy (LSC; 88,166 bp) and short single copy (SSC; 13,486 bp) regions flanked by inverted repeats (IR; 30,916 bp each) typical for angiosperms. The plastome includes 131 genes comprising 83 protein-coding genes, 37 transfer RNA genes, eight ribosomal RNA genes, two possible pseudogenes, psbL and rpl23 with internal stop codons, and truncated repeats of ndhF and rps19 at IR boundaries.
摘要:
我们组装了温带的塑性体,南半球lianaMuehlenbeckiaaustralis来自从总基因组DNA测序文库产生的高通量测序数据(配对末端Illumina读数)。澳大利亚分枝杆菌叶绿体基因组序列(GenBank:MG604297)长度为163,484bp,由长的单拷贝(LSC;88,166bp)和短的单拷贝(SSC;13,486bp)区域组成,两侧是被子植物典型的反向重复序列(IR;每个30,916bp)。质体包括131个基因,包含83个蛋白质编码基因,37个转移RNA基因,八个核糖体RNA基因,两种可能的伪基因,带内部终止密码子的psbL和rpl23,以及在IR边界处的ndhF和rps19的截短重复。
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