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Abstract：
The presence of epithelial and connective tissue attachment at the peri-implant-soft tissue region has been demonstrated to provide a biological barrier of the alveolar bone from the oral environment. This barrier can be improved via surface modification of implant abutment materials. The effect of photofunctionalization on creating a bioactive surface for the enhancement of the epithelial and connective tissue attachment of zirconia implant abutment\'s peri-implant mucosal interface using organotypic model has not been investigated. Therefore, this study aimed to evaluate the soft tissue seal around peri-implant mucosa and to understand the effect of photofunctionalization on the abutment materials. Three types of abutment materials were used in this study; yttria-stabilized zirconia (YSZ), alumina-toughened zirconia, and grade 2 commercially pure titanium (CPTi) which were divided into nontreated (N-Tx) and photofunctionalized group (UV-Tx). The three-dimensional peri-implant mucosal model was constructed using primary human gingival keratinocytes and fibroblasts co-cultured on the acellular dermal membrane. The biological seal was determined through the concentration of tritiated water permeating the material-soft tissue interface. The biological seal formed by the soft tissue in the N-Tx group was significantly reduced compared to the UV-treated group (p < 0.001), with YSZ exhibiting the lowest permeability among all materials. Photofunctionalization of implant abutment materials improved the biological seal of the surrounding soft tissue peri-implant interface.
摘要：
已证明在植入物周围软组织区域存在上皮和结缔组织附着，可提供牙槽骨与口腔环境的生物屏障。这种屏障可以通过种植体基牙材料的表面改性来改善。尚未研究使用器官模型的光功能化对形成生物活性表面以增强氧化锆植入物基台的种植体周围粘膜界面的上皮和结缔组织附着的影响。因此,本研究旨在评估种植体周围粘膜周围的软组织密封，并了解光功能化对基台材料的影响。本研究使用了三种类型的基台材料；氧化钇稳定的氧化锆(YSZ)，氧化铝增韧氧化锆,和2级商业纯钛（CPTi），分为未处理（N-Tx）和光官能化基团（UV-Tx）。使用在无细胞真皮膜上共培养的原代人牙龈角质形成细胞和成纤维细胞构建三维植入物周围粘膜模型。通过渗透材料-软组织界面的tri化水的浓度来确定生物密封。与UV处理组相比,N-Tx组软组织形成的生物密封显著减少(p<0.001),YSZ在所有材料中表现出最低的渗透率。种植体基台材料的光功能化改善了周围软组织种植体周围界面的生物密封。
