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Abstract：
Antibacterial scaffolds are highly desirable for the repair and reconstruction of injured soft tissues. However, the direct fabrication of scaffolds with excellent biocompatibility, flexibility, and antibacterial capacity remains a challenge, especially those based on biomaterials. In this study, we report the biomaterial-based antibacterial scaffolds based on regenerated silk fibroin, 2-hydroxypropyltrimethyl ammonium chloride chitosan, and bladder acellular matrix graft by blend and coaxial electrospinning. This approach eliminated the use of organic solvents and inorganic nanoparticles, ensuring greater clinical safety, mimicking physiological extracellular matrix structures, and the required softness for a suture material. Thus, the scaffold obtained in this study exhibited excellent biocompatibility, the required mechanical characteristics, and excellent antibacterial capacity. The rate of bacterial elimination of Staphylococcus aureus and Escherichia coli reached up to 99.5 and 98.3%, respectively. The scaffold design favored cell growth and proliferation and resulted in the significant promotion of repair and reconstruction of the urethra, indicating that it can be an ideal antibacterial suture material for soft tissue restoration.
摘要：
抗菌支架对于损伤的软组织的修复和重建是非常理想的。然而,直接制造具有优异生物相容性的支架，灵活性,抗菌能力仍然是一个挑战，尤其是那些基于生物材料的。在这项研究中,我们报道了基于再生丝素蛋白的生物材料抗菌支架，2-羟丙基三甲基氯化铵壳聚糖，并通过共混和同轴静电纺丝进行膀胱脱细胞基质移植。这种方法消除了有机溶剂和无机纳米颗粒的使用，确保更大的临床安全，模仿生理细胞外基质结构，和缝合材料所需的柔软度。因此，在这项研究中获得的支架表现出优异的生物相容性，所需的机械特性，和优良的抗菌能力。金黄色葡萄球菌和大肠杆菌的细菌消除率分别达到99.5%和98.3%,分别。支架设计有利于细胞的生长和增殖，对尿道的修复和重建有显著的促进作用，表明它可以是软组织修复的理想抗菌缝合材料。
