PDF(Sci-hub)
Abstract：
BACKGROUND: 5-aminolevulinic acid mediated photodynamic therapy (ALA-PDT) is increasingly used to control severe acne. However, its impact on skin microbiota remains uncertain.
OBJECTIVE: We aimed to compare the makeup, diversity, and function of the microbiota in pilosebaceous units of patients with severe acne before and after ALA-PDT.
METHODS: A longitudinal cohort study was performed on 11 participants with severe facial acne. All patients were given 5%ALA-PDT every two weeks for three sessions in total. The contents of lesions were sampled for metagenomic sequencing at baseline and two weeks after the first ALA-PDT session.
RESULTS: Cutibacterium acnes was the most dominant species followed by Staphylococcus epidermidis and Pseudomonas fluorescens. Treatment with ALA-PDT led to clinical improvements in acne severity concurrent with a significant reduction in the relative abundance of C. acnes, while P. fluorescens increased significantly after ALA-PDT. No significant change was identified in other species. ALA-PDT administration was associated with an increased microbiota diversity and reductions in the relative abundance of the functional genes involved in energy metabolism and DNA replication.
CONCLUSIONS: ALA-PDT plays a therapeutic role by killing C. acnes, increasing P. fluorescens and the microbiome diversity, while inhibiting the function of microbiota in pilosebaceous units of severe acne.
OBJECTIVE: We aimed to compare the makeup, diversity, and function of the microbiota in pilosebaceous units of patients with severe acne before and after ALA-PDT.
METHODS: A longitudinal cohort study was performed on 11 participants with severe facial acne. All patients were given 5%ALA-PDT every two weeks for three sessions in total. The contents of lesions were sampled for metagenomic sequencing at baseline and two weeks after the first ALA-PDT session.
RESULTS: Cutibacterium acnes was the most dominant species followed by Staphylococcus epidermidis and Pseudomonas fluorescens. Treatment with ALA-PDT led to clinical improvements in acne severity concurrent with a significant reduction in the relative abundance of C. acnes, while P. fluorescens increased significantly after ALA-PDT. No significant change was identified in other species. ALA-PDT administration was associated with an increased microbiota diversity and reductions in the relative abundance of the functional genes involved in energy metabolism and DNA replication.
CONCLUSIONS: ALA-PDT plays a therapeutic role by killing C. acnes, increasing P. fluorescens and the microbiome diversity, while inhibiting the function of microbiota in pilosebaceous units of severe acne.
摘要：
背景：5-氨基乙酰丙酸介导的光动力疗法（ALA-PDT）越来越多地用于控制重度痤疮。然而,其对皮肤微生物群的影响仍不确定。
目的：我们的目的是比较妆容，多样性,ALA-PDT前后严重痤疮患者毛囊皮脂腺单位微生物群的功能。
方法：对11名患有严重面部痤疮的参与者进行了一项纵向队列研究。所有患者每两周给予5%ALA-PDT,共三个疗程。在基线和第一次ALA-PDT疗程后两周对损伤的内容物进行取样用于宏基因组测序。
结果：粉刺杆菌是最主要的菌种，其次是表皮葡萄球菌和荧光假单胞菌。ALA-PDT治疗导致痤疮严重程度的临床改善，同时痤疮梭菌的相对丰度显着降低，而荧光假单胞菌在ALA-PDT后显著增加。在其他物种中未发现显着变化。ALA-PDT给药与微生物群多样性增加以及与能量代谢和DNA复制有关的功能基因的相对丰度降低有关。
结论：ALA-PDT通过杀死痤疮梭菌发挥治疗作用，增加荧光假单胞菌和微生物组多样性，同时抑制严重痤疮毛囊皮脂腺单位中微生物群的功能。
目的：我们的目的是比较妆容，多样性,ALA-PDT前后严重痤疮患者毛囊皮脂腺单位微生物群的功能。
方法：对11名患有严重面部痤疮的参与者进行了一项纵向队列研究。所有患者每两周给予5%ALA-PDT,共三个疗程。在基线和第一次ALA-PDT疗程后两周对损伤的内容物进行取样用于宏基因组测序。
结果：粉刺杆菌是最主要的菌种，其次是表皮葡萄球菌和荧光假单胞菌。ALA-PDT治疗导致痤疮严重程度的临床改善，同时痤疮梭菌的相对丰度显着降低，而荧光假单胞菌在ALA-PDT后显著增加。在其他物种中未发现显着变化。ALA-PDT给药与微生物群多样性增加以及与能量代谢和DNA复制有关的功能基因的相对丰度降低有关。
结论：ALA-PDT通过杀死痤疮梭菌发挥治疗作用，增加荧光假单胞菌和微生物组多样性，同时抑制严重痤疮毛囊皮脂腺单位中微生物群的功能。
