关键词: Biofilm Fluorometry Guidelines Microplate Reproducibility Spectrophotometry

来  源:   DOI:10.1016/j.bioflm.2019.100010   PDF(Sci-hub)   PDF(Pubmed)

Abstract:
The lack of reproducibility of published studies is one of the major issues facing the scientific community, and the field of biofilm microbiology has been no exception. One effective strategy against this multifaceted problem is the use of minimum information guidelines. This strategy provides a guide for authors and reviewers on the necessary information that a manuscript should include for the experiments in a study to be clearly interpreted and independently reproduced. As a result of several discussions between international groups working in the area of biofilms, we present a guideline for the spectrophotometric and fluorometric assessment of biofilm formation in microplates. This guideline has been divided into 5 main sections, each presenting a comprehensive set of recommendations. The intention of the minimum information guideline is to improve the quality of scientific communication that will augment interlaboratory reproducibility in biofilm microplate assays.
摘要:
发表的研究缺乏可重复性是科学界面临的主要问题之一,生物膜微生物学领域也不例外。解决这一多方面问题的一个有效策略是使用最低限度的信息准则。该策略为作者和审稿人提供了有关手稿应包含的必要信息的指南,以清楚地解释和独立复制研究中的实验。由于在生物膜领域工作的国际小组之间进行了多次讨论,我们提出了一个指南的分光光度和荧光评估生物膜形成的微孔板。本指南分为五个主要部分,每个人都提出了一套全面的建议。最低信息指南的目的是提高科学交流的质量,这将增强生物膜微孔板测定中的实验室间可重复性。
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