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Abstract：
In zebrafish, lymphatic endothelial cells (LECs) originate from multiple/several distinct progenitor populations and generate organ-specific lymphatic vasculatures. Cell fate and tissue specificities were determined using a combination of genetically engineered transgenic lines in which the promoter of a LEC-specific gene drives expression of a fluorescent reporter protein.
We established a novel zebrafish transgenic line expressing eGFP under the control of part of the zebrafish batf3 promoter (Basic Leucine Zipper ATF-Like Transcription Factor 3). Spatiotemporal examination of Tg(batf3MIN:eGFP) transgenic fish revealed a typical lymphatic expression pattern, which does not perfectly recapitulate the expression pattern of existing LEC transgenic lines. eGFP+ cells constitute a heterogeneous endothelial cell population, which expressed LEC and/or blood endothelial cells (BEC) markers in different tissues. In addition, we characterize the renal eGFP+ cell as a population of interest to study kidney diseases and regeneration.
Our Tg(batf3MIN:eGFP) reporter zebrafish line provides a useful system to study LEC populations, of which heterogeneity depends on origin of progenitors, tissue environment and physiological conditions. We further developed a novel fish-adapted tissue clearing method, which allows deep imaging and 3D-visualization of vascular and lymphatic networks in the whole organism.
We established a novel zebrafish transgenic line expressing eGFP under the control of part of the zebrafish batf3 promoter (Basic Leucine Zipper ATF-Like Transcription Factor 3). Spatiotemporal examination of Tg(batf3MIN:eGFP) transgenic fish revealed a typical lymphatic expression pattern, which does not perfectly recapitulate the expression pattern of existing LEC transgenic lines. eGFP+ cells constitute a heterogeneous endothelial cell population, which expressed LEC and/or blood endothelial cells (BEC) markers in different tissues. In addition, we characterize the renal eGFP+ cell as a population of interest to study kidney diseases and regeneration.
Our Tg(batf3MIN:eGFP) reporter zebrafish line provides a useful system to study LEC populations, of which heterogeneity depends on origin of progenitors, tissue environment and physiological conditions. We further developed a novel fish-adapted tissue clearing method, which allows deep imaging and 3D-visualization of vascular and lymphatic networks in the whole organism.
摘要：
在斑马鱼中,淋巴内皮细胞（LEC）起源于多个/几个不同的祖细胞群，并产生器官特异性淋巴管。使用基因工程改造的转基因系的组合确定细胞命运和组织特异性,其中LEC特异性基因的启动子驱动荧光报告蛋白的表达。
我们建立了在斑马鱼batf3启动子（碱性亮氨酸拉链ATF样转录因子3）的部分控制下表达eGFP的新型斑马鱼转基因系。Tg（batf3MIN：eGFP）转基因鱼的时空检查揭示了典型的淋巴表达模式，这并不能完美地概括现有LEC转基因品系的表达模式。eGFP+细胞构成异质内皮细胞群,其在不同组织中表达LEC和/或血液内皮细胞(BEC)标志物。此外,我们将肾eGFP+细胞表征为研究肾脏疾病和再生的感兴趣群体。
我们的Tg（batf3MIN：eGFP）报告斑马鱼品系提供了一个有用的系统来研究LEC种群，其异质性取决于祖细胞的起源，组织环境和生理条件。我们进一步开发了一种新的适应鱼的组织清除方法，它可以对整个生物体中的血管和淋巴网络进行深度成像和3D可视化。
我们建立了在斑马鱼batf3启动子（碱性亮氨酸拉链ATF样转录因子3）的部分控制下表达eGFP的新型斑马鱼转基因系。Tg（batf3MIN：eGFP）转基因鱼的时空检查揭示了典型的淋巴表达模式，这并不能完美地概括现有LEC转基因品系的表达模式。eGFP+细胞构成异质内皮细胞群,其在不同组织中表达LEC和/或血液内皮细胞(BEC)标志物。此外,我们将肾eGFP+细胞表征为研究肾脏疾病和再生的感兴趣群体。
我们的Tg（batf3MIN：eGFP）报告斑马鱼品系提供了一个有用的系统来研究LEC种群，其异质性取决于祖细胞的起源，组织环境和生理条件。我们进一步开发了一种新的适应鱼的组织清除方法，它可以对整个生物体中的血管和淋巴网络进行深度成像和3D可视化。
