We established a novel zebrafish transgenic line expressing eGFP under the control of part of the zebrafish batf3 promoter (Basic Leucine Zipper ATF-Like Transcription Factor 3). Spatiotemporal examination of Tg(batf3MIN:eGFP) transgenic fish revealed a typical lymphatic expression pattern, which does not perfectly recapitulate the expression pattern of existing LEC transgenic lines. eGFP+ cells constitute a heterogeneous endothelial cell population, which expressed LEC and/or blood endothelial cells (BEC) markers in different tissues. In addition, we characterize the renal eGFP+ cell as a population of interest to study kidney diseases and regeneration.
Our Tg(batf3MIN:eGFP) reporter zebrafish line provides a useful system to study LEC populations, of which heterogeneity depends on origin of progenitors, tissue environment and physiological conditions. We further developed a novel fish-adapted tissue clearing method, which allows deep imaging and 3D-visualization of vascular and lymphatic networks in the whole organism.
我们建立了在斑马鱼batf3启动子(碱性亮氨酸拉链ATF样转录因子3)的部分控制下表达eGFP的新型斑马鱼转基因系。Tg(batf3MIN:eGFP)转基因鱼的时空检查揭示了典型的淋巴表达模式,这并不能完美地概括现有LEC转基因品系的表达模式。eGFP+细胞构成异质内皮细胞群,其在不同组织中表达LEC和/或血液内皮细胞(BEC)标志物。此外,我们将肾eGFP+细胞表征为研究肾脏疾病和再生的感兴趣群体。
我们的Tg(batf3MIN:eGFP)报告斑马鱼品系提供了一个有用的系统来研究LEC种群,其异质性取决于祖细胞的起源,组织环境和生理条件。我们进一步开发了一种新的适应鱼的组织清除方法,它可以对整个生物体中的血管和淋巴网络进行深度成像和3D可视化。