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Abstract：
UNASSIGNED: To investigate the role of the periotic mesenchyme (POM) in the development of sensory cells of developing auditory epithelium.
UNASSIGNED: Developing auditory epithelium with or without periotic mesenchyme was isolated from mice at embryonic days 11.5 (E11.5), E12.5 and E13.5, respectively, and cultured in vitro to an equivalent of E18.5\'s epithelium in vivo. Then, the explants were co-stained with antibodies targeting myosin VIIA, Sox2 and BrdU.
UNASSIGNED: More hair cells in E11.5 + 7 DIV, E12.5 + 6 DIV and E13.5 + 5 DIV auditory epithelia were found upon culture with POM (225.90 ± 62.44, 476.94 ± 100.81, and 1386.60 ± 202.38, respectively) compared with the non-POM group (68.17 ± 23.74, 205.00 ± 44.23, and 1266.80 ± 38.84, respectively). Moreover, regardless of developmental stage, the mesenchymal tissue increased the amount of cochlear sensory cells as well as the ratio of differentiated hair cells to total sensory cells.
UNASSIGNED: The periotic mesenchyme promotes the development of cochlear sensory cells, and its effect depends on the developmental stage of the auditory epithelium.
UNASSIGNED: Developing auditory epithelium with or without periotic mesenchyme was isolated from mice at embryonic days 11.5 (E11.5), E12.5 and E13.5, respectively, and cultured in vitro to an equivalent of E18.5\'s epithelium in vivo. Then, the explants were co-stained with antibodies targeting myosin VIIA, Sox2 and BrdU.
UNASSIGNED: More hair cells in E11.5 + 7 DIV, E12.5 + 6 DIV and E13.5 + 5 DIV auditory epithelia were found upon culture with POM (225.90 ± 62.44, 476.94 ± 100.81, and 1386.60 ± 202.38, respectively) compared with the non-POM group (68.17 ± 23.74, 205.00 ± 44.23, and 1266.80 ± 38.84, respectively). Moreover, regardless of developmental stage, the mesenchymal tissue increased the amount of cochlear sensory cells as well as the ratio of differentiated hair cells to total sensory cells.
UNASSIGNED: The periotic mesenchyme promotes the development of cochlear sensory cells, and its effect depends on the developmental stage of the auditory epithelium.
摘要：
■研究周围间质（POM）在发育中的听觉上皮的感觉细胞发育中的作用。
■在胚胎期11.5（E11.5）从小鼠中分离出发育中的有或没有周围间质的听觉上皮，分别为E12.5和E13.5，并在体外培养到相当于E18.5的体内上皮。然后，外植体与靶向肌球蛋白VIIA的抗体共染色，Sox2和BrdU。
■E11.5+7DIV中的更多毛细胞，与非POM组（68.17±23.74，205.00±44.23和1266.80±38.84）相比，POM培养时发现了E12.56DIV和E13.55DIV听觉上皮（分别为225.90±62.44、476.94±100.81和1386.60±202.38）。此外，无论发展阶段如何，间充质组织增加了耳蜗感觉细胞的数量以及分化的毛细胞与总感觉细胞的比例。
■周围间质促进耳蜗感觉细胞的发育，其效果取决于听觉上皮的发育阶段。
■在胚胎期11.5（E11.5）从小鼠中分离出发育中的有或没有周围间质的听觉上皮，分别为E12.5和E13.5，并在体外培养到相当于E18.5的体内上皮。然后，外植体与靶向肌球蛋白VIIA的抗体共染色，Sox2和BrdU。
■E11.5+7DIV中的更多毛细胞，与非POM组（68.17±23.74，205.00±44.23和1266.80±38.84）相比，POM培养时发现了E12.56DIV和E13.55DIV听觉上皮（分别为225.90±62.44、476.94±100.81和1386.60±202.38）。此外，无论发展阶段如何，间充质组织增加了耳蜗感觉细胞的数量以及分化的毛细胞与总感觉细胞的比例。
■周围间质促进耳蜗感觉细胞的发育，其效果取决于听觉上皮的发育阶段。
