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Abstract：
Recently, dental pulp has been attracting attention as a promising source of multipotent mesenchymal stem cells (MSCs) for various clinical applications of regeneration fields. To date, we have succeeded in establishing rat dental pulp-derived cells showing the characteristics of odontoblasts under in vitro conditions. We named them Tooth matrix-forming, GFP rat-derived Cells (TGC). However, though TGC form massive dentin-like hard tissues under in vivo conditions, this does not lead to the induction of polar odontoblasts. Focusing on the importance of the geometrical structure of an artificial biomaterial to induce cell differentiation and hard tissue formation, we previously have succeeded in developing a new biomaterial, honeycomb tricalcium phosphate (TCP) scaffold with through-holes of various diameters. In this study, to induce polar odontoblasts, TGC were induced to form odontoblasts using honeycomb TCP that had various hole diameters (75, 300, and 500 μm) as a scaffold. The results showed that honeycomb TCP with 300-μm hole diameters (300TCP) differentiated TGC into polar odontoblasts that were DSP positive. Therefore, our study indicates that 300TCP is an appropriate artificial biomaterial for dentin regeneration.
摘要：
最近,牙髓作为多能间充质干细胞（MSCs）的有希望的来源，在再生领域的各种临床应用中受到关注。迄今为止,我们已经成功地建立了在体外条件下显示成牙本质细胞特征的大鼠牙髓来源的细胞。我们把它们命名为牙齿矩阵形成，GFP大鼠来源的细胞(TGC)。然而,尽管TGC在体内条件下形成大量牙本质样的硬组织，这不会导致极性成牙本质细胞的诱导。关注人造生物材料的几何结构对诱导细胞分化和硬组织形成的重要性，我们以前成功地开发了一种新的生物材料，蜂窝状磷酸三钙（TCP）支架，具有各种直径的通孔。在这项研究中,诱导极性成牙本质细胞，使用具有各种孔直径（75、300和500μm）的蜂窝状TCP作为支架，诱导TGC形成成牙本质细胞。结果表明，孔径为300μm的蜂窝状TCP（300TCP）将TGC区分为DSP阳性的极性成牙本质细胞。因此,我们的研究表明，300TCP是一种合适的用于牙本质再生的人工生物材料。
