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Abstract：
Hydatidosis is one of the most important zoonotic diseases with a worldwide distribution and it seems that the survival of Echinococcus granulosus in nature for many years, is due to having different mechanisms to escape from the host immune systems. One of these efficient mechanisms is the production of various antigens and proteins by the larva of the parasite and the main purpose of this study is evaluation of manifestation of various antigens in different parts of intermediate host. The hepatic and pulmonary hydatid cysts were gathered from sheep and the antigens of different parts of the cysts (laminated layer, protoscolices and cyst fluid) were separated and analyzed by SDS-PAGE and then transferred to nitrocellulose paper and finally, Western blot analysis was evaluated the immunogenicity of proteins. The antigens of laminated layer, protoscolices and hydatid cyst fluid, in different tissues of the liver and lungs, manifest various proteins and also these antigens are immunogenically different. Also, it is found more immunogenic proteins in the laminated layer than the other parts of the cysts. The various proteins are generated by Echinococcus granulosus larva depending on the type of tissues attacked by the parasite. Increasing the chance of survival may be the main cause of manifestation various antigens in different parts of cysts and host tissues. These antigenic variations might have made diagnostic serologic test unreliable.
摘要：
包虫病是世界范围内分布的最重要的人畜共患疾病之一,细粒棘球蚴在自然界中存活多年,是由于有不同的机制来逃避宿主的免疫系统。这些有效机制之一是寄生虫幼虫产生各种抗原和蛋白质，这项研究的主要目的是评估中间宿主不同部分中各种抗原的表现。从绵羊和不同部位的抗原中收集肝和肺包虫囊肿（层压层，原液和囊液）通过SDS-PAGE分离和分析，然后转移到硝酸纤维素纸上，最后，蛋白质印迹分析评估蛋白质的免疫原性。层压层的抗原，原头囊和包虫囊肿液，在肝脏和肺的不同组织中，显示各种蛋白质和这些抗原是免疫原性不同的。此外，与囊肿的其他部分相比，在层压层中发现了更多的免疫原性蛋白。各种蛋白质由细粒棘球蚴幼虫产生，具体取决于寄生虫攻击的组织类型。增加存活机会可能是在囊肿和宿主组织的不同部位表现出各种抗原的主要原因。这些抗原变异可能使诊断血清学测试不可靠。
