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Abstract：
Glycosylation of the reproductive tract of an adult female red-necked ostrich (Struthio camelus camelus) carrying a fully formed calcified egg in her uterus when accidently killed by a blow to the head was examined using lectin histochemistry on samples from the infundibulum, magnum, uterus and vagina. Glycans in the luminal epithelium and underlying glands were described after staining with 23 lectins after neuraminidase pre-treatment in some cases. Ciliated and non-ciliated cells were evident at all levels in the luminal epithelium, the latter full of richly glycosylated secretory granules. The ciliated cells also showed glycosylation and, in the magnum, these cells often stained more intensely than the non-ciliated cells. High mannose and complex N-glycans, α1,6-linked fucosyl and sialic acid residues were present throughout the tract and there was a complete absence of GalNAcα1,3(LFucα1,2)Galß1,3/4GlcNAcß1- and rare terminal GalNAcα1- residues. Fucose in α1,2-linkage as H2 antigen and Ley was also rare in the luminal epithelium and completely absent in glands. Terminal galactose was present in the luminal epithelium apart from in the infundibulum. Gland epithelium showed similar glycosylation to the luminal epithelium except in the magnum where there were significant differences and here the glands were packed full of large secretory granules, unlike the glands in the rest of the tract. Each section of the tract had its own specific pattern of glycosylation which could be related to the stage of egg formation.
摘要：
成年雌性红颈鸵鸟（Struthiocameluscamelus）的生殖道糖基化，当意外地将头部撞死时，在子宫中携带完全形成的钙化卵时，使用凝集素组织化学对漏斗样本进行了检查，大号,子宫和阴道.在某些情况下，在神经氨酸酶预处理后，用23种凝集素染色后，描述了腔上皮和下面腺体中的聚糖。纤毛和非纤毛细胞在腔上皮的所有水平都很明显，后者充满了丰富的糖基化分泌颗粒。纤毛细胞也表现出糖基化，在大号，这些细胞通常比非纤毛细胞染色更强烈。高甘露糖和复杂的N-聚糖，α1,6-连接的岩藻糖基和唾液酸残基存在于整个管道中，并且完全不存在GalNAcα1,3（LFuca1,2）Galβ1,3/4GlcNAc1-和稀有末端GalNAcα1-残基。α1,2-连接的岩藻糖作为H2抗原和Ley在腔上皮中也很少见，在腺体中完全不存在。除漏斗外，末端半乳糖存在于管腔上皮中。腺体上皮表现出与腔上皮相似的糖基化，除了在大细胞中存在显着差异，并且在这里腺体充满了大的分泌颗粒，不像其余的腺体.管道的每个部分都有自己特定的糖基化模式，这可能与卵的形成阶段有关。
