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Abstract：
Herein, we report a novel sensor to detect trypsin using a purpose-designed fluorescein-labelled peptide with negatively charged carbon nanoparticles (CNPs) modified by acid oxidation. The fluorescence of the fluorescein-labelled peptide was quenched by CNPs. The sensor reacted with trypsin to cleave the peptide, resulting in the release of the dye moiety and a substantial increase in fluorescence intensity, which was dose- and time-dependent, and trypsin could be quantified accordingly. Correspondingly, the biosensor has led to the development of a convenient and efficient fluorescent method to measure trypsin activity, with a detection limit of 0.7 μg/mL. The method allows rapid determination of trypsin activity in the normal and acute pancreatitis range, suitable for point-of-care testing. Furthermore, the applicability of the method has been demonstrated by detecting trypsin in spiked urine samples.
摘要：
在这里，我们报告了一种新型传感器，使用专门设计的荧光素标记的肽检测胰蛋白酶，该肽具有通过酸氧化修饰的带负电荷的碳纳米颗粒（CNPs）。荧光素标记的肽的荧光被CNP猝灭。传感器与胰蛋白酶反应以切割肽，导致染料部分的释放和荧光强度的大幅增加，这是剂量和时间依赖性的，和胰蛋白酶可以相应地定量。相应地,生物传感器导致了一种方便高效的荧光法来测量胰蛋白酶活性的发展，检测限为0.7μg/mL。该方法可以快速测定正常和急性胰腺炎范围内的胰蛋白酶活性，适用于即时测试。此外，通过检测加标尿液样品中的胰蛋白酶，证明了该方法的适用性。
