Abstract:
Morphologically controllable synthesis of Rh nanoparticles (NPs) was achieved by the use of additives during polyol synthesis. The effect of salts and surfactant additives including PVP, sodium acetate, sodium citrate, CTAB, CTAC, and potassium bromide on Rh NPs morphology was investigated. When PVP was used as the only additive, trigonal NPs were obtained. Additives containing Br- ions (CTAB and KBr) resulted in NPs with a cubic morphology, while those with carboxyl groups (sodium citrate and acetate) formed spheroid NPs. The use of Cl- ions (CTAC) resulted in a mixture of polygon morphologies. Cytotoxicity of these NPs was evaluated on macrophages and ovarian cancer cell lines. Membrane integrity and cellular activity are both influenced to a similar extent, for both the cell lines, with respect to the morphology of Rh NPs. The cells exposed to trigonal Rh NPs showed the highest viability, among the NP series. Particles with a mixed polygon morphology had the highest cytotoxic impact, followed by cubic and spherical NPs. The Rh NPs were further demonstrated as contrast agents for X-ray fluorescence computed tomography (XFCT) in a small-animal imaging setting. This work provides a detailed route for the synthesis, morphology control, and characterization of Rh NPs as viable contrast agents for XFCT bio-imaging.
摘要:
通过在多元醇合成过程中使用添加剂实现Rh纳米颗粒(NPs)的形态可控合成。盐和表面活性剂添加剂(包括PVP)的作用,乙酸钠,柠檬酸钠,CTAB,CTAC,研究了溴化钾对RhNPs形态的影响。当PVP被用作唯一的添加剂时,获得了三角NPs。含有Br-离子的添加剂(CTAB和KBr)导致具有立方形态的NP,而具有羧基的那些(柠檬酸钠和醋酸盐)形成球形NP。Cl-离子(CTAC)的使用导致多边形形态的混合。在巨噬细胞和卵巢癌细胞系上评估这些NP的细胞毒性。膜完整性和细胞活性都受到相似程度的影响,对于两种细胞系,关于RhNP的形态。暴露于三角RhNP的细胞显示出最高的活力,在NP系列中。具有混合多边形形态的颗粒具有最高的细胞毒性影响,其次是立方和球形NP。在小动物成像环境中,RhNP进一步被证明是X射线荧光计算机断层扫描(XFCT)的造影剂。这项工作为合成提供了详细的路线,形态控制,以及RhNP作为XFCT生物成像可行造影剂的表征。