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Abstract：
UNASSIGNED: Visceral fat is an independent risk factor for metabolic and cardiovascular disease. The study aimed to investigate the associations between gut microbiome and visceral fat.
UNASSIGNED: We recruited 32 obese adults and 30 healthy controls at baseline. Among the obese subjects, 14 subjects underwent laparoscopic sleeve gastrectomy (LSG) and were followed 6 months after surgery. Abdominal visceral fat area (VFA) and subcutaneous fat area (SFA) were measured by magnetic resonance imaging. Waist, hipline, waist-to-hip ratio (WHR) and body mass index (BMI) were included as simple obese parameters. Gut microbiome was analyzed by metagenomic sequencing.
UNASSIGNED: Among the obese parameters, VFA had the largest number of correlations with the species that were differentially enriched between obese and healthy subjects, following by waist, WHR, BMI, hipline, and SFA. Within the species negatively correlated with VFA, Eubacterium eligens had the strongest correlation, following by Clostridium citroniae, C. symbiosum, Bacteroides uniformis, E. ventriosum, Ruminococcaceae bacterium D16, C. hathewayi, etc. C. hathewayi and C. citroniae were increased after LSG. Functional analyses showed that among all the obese parameters, VFA had strongest correlation coefficients with the obesity-related microbial pathways. Microbial pathways involved in carbohydrate fermentation and biosynthesis of L-glutamate and L-glutamine might contribute to visceral fat accumulation.
UNASSIGNED: Visceral fat was more closely correlated with gut microbiome compared with subcutaneous fat, suggesting an intrinsic connection between gut microbiome and metabolic cardiovascular diseases. Specific microbial species and pathways which were closely associated with visceral fat accumulation might contribute to new targeted therapies for metabolic disorders.
UNASSIGNED: We recruited 32 obese adults and 30 healthy controls at baseline. Among the obese subjects, 14 subjects underwent laparoscopic sleeve gastrectomy (LSG) and were followed 6 months after surgery. Abdominal visceral fat area (VFA) and subcutaneous fat area (SFA) were measured by magnetic resonance imaging. Waist, hipline, waist-to-hip ratio (WHR) and body mass index (BMI) were included as simple obese parameters. Gut microbiome was analyzed by metagenomic sequencing.
UNASSIGNED: Among the obese parameters, VFA had the largest number of correlations with the species that were differentially enriched between obese and healthy subjects, following by waist, WHR, BMI, hipline, and SFA. Within the species negatively correlated with VFA, Eubacterium eligens had the strongest correlation, following by Clostridium citroniae, C. symbiosum, Bacteroides uniformis, E. ventriosum, Ruminococcaceae bacterium D16, C. hathewayi, etc. C. hathewayi and C. citroniae were increased after LSG. Functional analyses showed that among all the obese parameters, VFA had strongest correlation coefficients with the obesity-related microbial pathways. Microbial pathways involved in carbohydrate fermentation and biosynthesis of L-glutamate and L-glutamine might contribute to visceral fat accumulation.
UNASSIGNED: Visceral fat was more closely correlated with gut microbiome compared with subcutaneous fat, suggesting an intrinsic connection between gut microbiome and metabolic cardiovascular diseases. Specific microbial species and pathways which were closely associated with visceral fat accumulation might contribute to new targeted therapies for metabolic disorders.
摘要：
■内脏脂肪是代谢和心血管疾病的独立危险因素。该研究旨在调查肠道微生物组和内脏脂肪之间的关联。
■我们在基线时招募了32名肥胖成年人和30名健康对照。在肥胖的受试者中，14例患者行腹腔镜袖状胃切除术（LSG），术后6个月随访。通过磁共振成像测量腹部内脏脂肪面积（VFA）和皮下脂肪面积（SFA）。腰部,hipline,腰臀比(WHR)和体重指数(BMI)作为单纯性肥胖参数.通过宏基因组测序分析肠道微生物组。
■在肥胖参数中，VFA与肥胖和健康受试者之间差异富集的物种的相关性最大，紧随腰部，WHR,BMI,hipline,SFA。在与VFA负相关的物种中，Eubacteriumeligins具有最强的相关性，其次是肺炎梭菌，C.共生，均匀拟杆菌,E.ventriosum,Ruminocycaceae细菌D16，C.hathewayi，等。LSG后C.hathewayi和c.citronium增加。功能分析显示，在所有肥胖参数中，VFA与肥胖相关的微生物途径具有最强的相关系数。参与碳水化合物发酵以及L-谷氨酸和L-谷氨酰胺的生物合成的微生物途径可能有助于内脏脂肪的积累。
■与皮下脂肪相比，内脏脂肪与肠道微生物组更密切相关，提示肠道微生物组和代谢性心血管疾病之间存在内在联系。与内脏脂肪积累密切相关的特定微生物种类和途径可能有助于新的靶向治疗代谢紊乱。
■我们在基线时招募了32名肥胖成年人和30名健康对照。在肥胖的受试者中，14例患者行腹腔镜袖状胃切除术（LSG），术后6个月随访。通过磁共振成像测量腹部内脏脂肪面积（VFA）和皮下脂肪面积（SFA）。腰部,hipline,腰臀比(WHR)和体重指数(BMI)作为单纯性肥胖参数.通过宏基因组测序分析肠道微生物组。
■在肥胖参数中，VFA与肥胖和健康受试者之间差异富集的物种的相关性最大，紧随腰部，WHR,BMI,hipline,SFA。在与VFA负相关的物种中，Eubacteriumeligins具有最强的相关性，其次是肺炎梭菌，C.共生，均匀拟杆菌,E.ventriosum,Ruminocycaceae细菌D16，C.hathewayi，等。LSG后C.hathewayi和c.citronium增加。功能分析显示，在所有肥胖参数中，VFA与肥胖相关的微生物途径具有最强的相关系数。参与碳水化合物发酵以及L-谷氨酸和L-谷氨酰胺的生物合成的微生物途径可能有助于内脏脂肪的积累。
■与皮下脂肪相比，内脏脂肪与肠道微生物组更密切相关，提示肠道微生物组和代谢性心血管疾病之间存在内在联系。与内脏脂肪积累密切相关的特定微生物种类和途径可能有助于新的靶向治疗代谢紊乱。
