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Abstract：
OBJECTIVE: An adequate storage medium can help preserve the viability of periodontal ligament cells, favoring the periodontal ligament repair process. The aim of this study was to evaluate the repair process following the delayed replantation of teeth stored in soy milk and whole milk.
METHODS: The maxillary right incisors of 27 rats were divided into three equally sized groups: Group SM (delayed replantation with storage in soy milk), whose teeth were immersed in 50 mL of soy milk for 60 minutes; Group WM (delayed replantation with whole milk), teeth were kept for 60 minutes in 50 mL of whole milk; and Group D (delayed replantation with dry storage), teeth were kept in dry conditions for the same period (negative control). Next, the teeth of the animals of all groups were replanted into their sockets. Systemic antibiotics were given to all the animals, and then, they were euthanized after 60 days. Sections were obtained and stained with hematoxylin and eosin for histomorphometric study. The presence and location of acute and chronic inflammatory processes in the epithelial insertion of the protective and insertion periodontium were evaluated, as well as the presence, extension, depth, and repair of root resorption, and the resorption and ankylosis with bone. Statistical differences between groups were analyzed using the Dunn test.
RESULTS: Groups SM and WM showed similar patterns in the repair of the periodontal ligament following delayed replantation, in relation to the inflammatory process in the proximities of the epithelial insertion, inflammation and organization of the periodontal ligament, plus root and bone resorption. However, in group WM, more ankylosis was observed than in group SM.
CONCLUSIONS: The teeth stored in soy milk and whole milk had similar periodontal repair, which shows the potential of soy milk as a storage medium before tooth replantation.
METHODS: The maxillary right incisors of 27 rats were divided into three equally sized groups: Group SM (delayed replantation with storage in soy milk), whose teeth were immersed in 50 mL of soy milk for 60 minutes; Group WM (delayed replantation with whole milk), teeth were kept for 60 minutes in 50 mL of whole milk; and Group D (delayed replantation with dry storage), teeth were kept in dry conditions for the same period (negative control). Next, the teeth of the animals of all groups were replanted into their sockets. Systemic antibiotics were given to all the animals, and then, they were euthanized after 60 days. Sections were obtained and stained with hematoxylin and eosin for histomorphometric study. The presence and location of acute and chronic inflammatory processes in the epithelial insertion of the protective and insertion periodontium were evaluated, as well as the presence, extension, depth, and repair of root resorption, and the resorption and ankylosis with bone. Statistical differences between groups were analyzed using the Dunn test.
RESULTS: Groups SM and WM showed similar patterns in the repair of the periodontal ligament following delayed replantation, in relation to the inflammatory process in the proximities of the epithelial insertion, inflammation and organization of the periodontal ligament, plus root and bone resorption. However, in group WM, more ankylosis was observed than in group SM.
CONCLUSIONS: The teeth stored in soy milk and whole milk had similar periodontal repair, which shows the potential of soy milk as a storage medium before tooth replantation.
摘要：
目的：适当的储存介质可以帮助保持牙周膜细胞的活力，有利于牙周膜修复过程。这项研究的目的是评估豆浆和全脂牛奶中储存的牙齿延迟再植后的修复过程。
方法：将27只大鼠的上颌右切牙分为3个大小相等的组：SM组（豆浆中储存的延迟再植），将其牙齿浸入50mL豆浆中60分钟；WM组（全脂牛奶延迟再植），将牙齿在50mL全脂牛奶中保持60分钟；和D组（干燥储存延迟再植），将牙齿保持在干燥条件下相同时间(阴性对照)。接下来,将所有组动物的牙齿重新植入它们的窝中。对所有动物给予全身抗生素，然后,他们在60天后被安乐死。获得切片并用苏木精和曙红染色以进行组织形态学研究。评估了保护性和插入性牙周组织的上皮插入中急性和慢性炎症过程的存在和位置，以及存在，扩展,深度,和修复根吸收，骨吸收和强直。使用Dunn检验分析组间的统计学差异。
结果：SM组和WM组在延迟再植后的牙周膜修复中显示出相似的模式，与上皮插入附近的炎症过程有关，牙周膜的炎症和组织，再加上根和骨吸收。然而,在WM组中，与SM组相比，观察到更多的强直。
结论：存放在豆浆和全脂牛奶中的牙齿具有相似的牙周修复，这表明豆浆作为牙齿再植前的储存介质的潜力。
方法：将27只大鼠的上颌右切牙分为3个大小相等的组：SM组（豆浆中储存的延迟再植），将其牙齿浸入50mL豆浆中60分钟；WM组（全脂牛奶延迟再植），将牙齿在50mL全脂牛奶中保持60分钟；和D组（干燥储存延迟再植），将牙齿保持在干燥条件下相同时间(阴性对照)。接下来,将所有组动物的牙齿重新植入它们的窝中。对所有动物给予全身抗生素，然后,他们在60天后被安乐死。获得切片并用苏木精和曙红染色以进行组织形态学研究。评估了保护性和插入性牙周组织的上皮插入中急性和慢性炎症过程的存在和位置，以及存在，扩展,深度,和修复根吸收，骨吸收和强直。使用Dunn检验分析组间的统计学差异。
结果：SM组和WM组在延迟再植后的牙周膜修复中显示出相似的模式，与上皮插入附近的炎症过程有关，牙周膜的炎症和组织，再加上根和骨吸收。然而,在WM组中，与SM组相比，观察到更多的强直。
结论：存放在豆浆和全脂牛奶中的牙齿具有相似的牙周修复，这表明豆浆作为牙齿再植前的储存介质的潜力。
