关键词: Cell wall degradation Cellulases Enzyme modeling Enzyme modification Enzyme-substrate interactions Glycosyl hidrolases Hemicellulases Ligninases Molecular dynamics Plant biomass management

来  源:   DOI:10.7717/peerj.9586   PDF(Pubmed)

Abstract:
Recycling biomass is indispensable these days not only because fossil energy sources are gradually depleted, but also because pollution of the environment, caused by the increasing use of energy, must be reduced. This article intends to overview the results of plant biomass processing methods that are currently in use. Our aim was also to review published methods that are not currently in use. It is intended to explore the possibilities of new methods and enzymes to be used in biomass recycling. The results of this overview are perplexing in almost every area. Advances have been made in the pre-treatment of biomass and in the diversity and applications of the enzymes utilized. Based on molecular modeling, very little progress has been made in the modification of existing enzymes for altered function and adaptation for the environmental conditions during the processing of biomass. There are hardly any publications in which molecular modeling techniques are used to improve enzyme function and to adapt enzymes to various environmental conditions. Our view is that using modern computational, biochemical, and biotechnological methods would enable the purposeful design of enzymes that are more efficient and suitable for biomass processing.
摘要:
如今,回收生物质是必不可少的,这不仅是因为化石能源逐渐枯竭,还因为环境污染,由于能源使用的增加,必须减少。本文拟对目前使用的植物生物质处理方法的结果进行综述。我们的目的还在于审查目前尚未使用的已发布方法。旨在探索用于生物质回收的新方法和酶的可能性。本概述的结果几乎在每个领域都令人困惑。在生物质的预处理以及所使用的酶的多样性和应用方面已经取得了进展。基于分子建模,在生物质加工过程中,在改变现有酶的功能和适应环境条件方面进展甚微。几乎没有任何出版物使用分子建模技术来改善酶功能并使酶适应各种环境条件。我们的观点是使用现代计算,生物化学,和生物技术方法将能够有目的地设计更有效和适用于生物质加工的酶。
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