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Abstract：
Whole-genome sequencing (WGS) of Treponema pallidum subspecies pallidum (TPA) has been constrained by the lack of in vitro cultivation methods for isolating spirochetes from patient samples.
We built upon recently developed enrichment methods to sequence TPA directly from primary syphilis chancre swabs collected in Guangzhou, China.
By combining parallel, pooled whole-genome amplification with hybrid selection, we generated high-quality genomes from 4 of 8 chancre-swab samples and 2 of 2 rabbit-passaged isolates, all subjected to challenging storage conditions.
This approach enabled the first WGS of Chinese samples without rabbit passage and provided insights into TPA genetic diversity in China.
We built upon recently developed enrichment methods to sequence TPA directly from primary syphilis chancre swabs collected in Guangzhou, China.
By combining parallel, pooled whole-genome amplification with hybrid selection, we generated high-quality genomes from 4 of 8 chancre-swab samples and 2 of 2 rabbit-passaged isolates, all subjected to challenging storage conditions.
This approach enabled the first WGS of Chinese samples without rabbit passage and provided insights into TPA genetic diversity in China.
摘要：
梅毒螺旋体亚种（TPA）的全基因组测序（WGS）受到缺乏从患者样品中分离螺旋体的体外培养方法的限制。
我们基于最近开发的富集方法，直接从广州收集的原发性梅毒下颌拭子中测序TPA，中国。
通过组合并行，混合全基因组扩增与杂种选择，我们从8个下颌拭子样本中的4个和2个兔传代分离株中的2个中产生了高质量的基因组，所有这些都受到具有挑战性的储存条件的影响。
这种方法实现了没有兔传代的中国样本的第一个WGS，并提供了对中国TPA遗传多样性的见解。
我们基于最近开发的富集方法，直接从广州收集的原发性梅毒下颌拭子中测序TPA，中国。
通过组合并行，混合全基因组扩增与杂种选择，我们从8个下颌拭子样本中的4个和2个兔传代分离株中的2个中产生了高质量的基因组，所有这些都受到具有挑战性的储存条件的影响。
这种方法实现了没有兔传代的中国样本的第一个WGS，并提供了对中国TPA遗传多样性的见解。
