RESULTS: In this study, we detected large genetic variation in a panel of 328 diverse barley (Hordeum vulgare L.) accessions. To understand the overall genetic basis of barley coleoptile length, all accessions were germinated in the dark and phenotyped for coleoptile length after 2 weeks. The investigated barleys had significant variation for coleoptile length. We then conducted genome-wide association studies (GWASs) with more than 30,000 molecular markers and identified 8 genes and 12 intergenic loci significantly associated with coleoptile length in our barley panel. The Squamosa promoter-binding-like protein 3 gene (SPL3) on chromosome 6H was identified as a major candidate gene. The missense variant on the second exon changed serine to alanine in the conserved SBP domain, which likely impacted its DNA-binding activity.
CONCLUSIONS: This study provides genetic loci for seedling coleoptile length along with candidate genes for future potential incorporation in breeding programmes to enhance early vigour and yield potential in water-limited environments.
结果:在这项研究中,我们在328个不同大麦(HordeumvulgareL.)种质中检测到了很大的遗传变异。了解大麦胚芽鞘长度的总体遗传基础,所有种质均在黑暗中发芽,并在2周后对胚芽鞘长度进行表型分析。调查的大麦胚芽鞘长度有明显差异。然后,我们使用30,000多个分子标记进行了全基因组关联研究(GWAS),并在大麦面板中鉴定了与胚芽鞘长度显着相关的8个基因和12个基因间基因座。6H染色体上的Squamosa启动子结合样蛋白3基因(SPL3)被鉴定为主要候选基因。第二个外显子上的错义变体在保守的SBP结构域中将丝氨酸改变为丙氨酸,这可能会影响其DNA结合活性。
结论:这项研究提供了幼苗胚芽鞘长度的遗传基因座,以及未来潜在的育种计划中的候选基因,以提高水分受限环境中的早期活力和产量潜力。