使用 UV 可激发染料和多个螯合剂头对在凝胶和膜中的多组氨酸标记的蛋白质的简化检测。
关键词: His-tag detection PAGE UV detection of His-tag protein UV transilluminator UVHis-PAGE Western blot blot membrane fluorescence histidine imaging metal-ion-chelating nitrilotriacetate (NTA) physical method recombinant protein expression
Mesh : Denaturing Gradient Gel Electrophoresis Fluorescent Dyes / chemistry Histidine / analysis chemistry Humans Recombinant Fusion Proteins / analysis chemistry Small Ubiquitin-Related Modifier Proteins / analysis chemistry genetics Ultraviolet Rays
来 源:
DOI:10.1074/jbc.RA120.014132
PDF(Sci-hub)
PDF(Pubmed)