PDF(Pubmed)
Abstract：
Adenoviruses are a frequent cause of acute upper respiratory tract infections that can also cause disseminated disease in immunosuppressed patients. We identified a novel adenovirus, squirrel monkey adenovirus 1 (SqMAdV-1), as the cause of fatal infection in an immunocompromised squirrel monkey (Saimiri boliviensis) at the Keeling Center for Comparative Medicine and Research (KCCMR). Sequencing of SqMAdV-1 revealed that it is most closely related (80.4 % pairwise nucleotide identity) to the titi monkey (Plecturocebus cupreus) adenovirus (TMAdV). Although identified in the titi monkey, TMAdV is highly lethal in these monkeys, and they are not thought to be the natural host. While SqMAdV-1 is similar to other primate adenoviruses in size and genomic characteristics, a nucleotide polymorphism at the expected stop codon of the DNA polymerase gene results in a 126 amino acid extension at the carboxy terminus, a feature not previously observed among other primate adenoviruses. PCR testing and partial sequencing of 95 archived faecal samples from other squirrel monkeys (Saimiri boliviensis and Saimiri sciureus) housed at the KCCMR revealed the presence of three distinct, and apparently endemic species of adenoviruses. A grouping of ten squirrel monkey adenovirus variants has high similarity to SqMAdV-1. A single adenovirus variant (designated SqMAdV-3), detected in five monkeys, has similarity to tufted capuchin (Sapajus apella) adenoviruses. The largest group of adenovirus variants detected (designated SqMAdV-2.0-2.16) has very high similarity (93-99 %) to the TMAdV, suggesting that squirrel monkeys may be the natural host of the TMAdV.
摘要：
腺病毒是急性上呼吸道感染的常见原因，也可能导致免疫抑制患者的播散性疾病。我们发现了一种新型腺病毒,松鼠猴腺病毒1(SqMAdV-1),作为基林比较医学和研究中心（KCCMR）的免疫功能低下的松鼠猴（Saimiriboliviensis）致命感染的原因。SqMAdV-1的测序显示，它与titi猴（Plecturocebuspupreus）腺病毒（TMAdV）最密切相关（成对核苷酸同一性为80.4％）。尽管在titi猴子中被识别，TMAdV在这些猴子中具有很高的致命性,他们不被认为是自然宿主。虽然SqMAdV-1在大小和基因组特征上与其他灵长类腺病毒相似，DNA聚合酶基因预期终止密码子处的核苷酸多态性导致羧基末端的126个氨基酸延伸，以前在其他灵长类腺病毒中没有观察到的特征。来自KCCMR的其他松鼠猴（Saimiriboliviensis和Saimirisciureus）的95个存档粪便样本的PCR测试和部分测序显示存在三种不同的情况，显然是腺病毒的特有物种。一组10个松鼠猴腺病毒变体与SqMAdV-1具有高度相似性。单个腺病毒变体（命名为SqMAdV-3），在五只猴子身上发现，与簇绒卷尾猴（Sapajusapella）腺病毒相似。检测到的最大一组腺病毒变体（命名为SqMAdV-2.0-2.16）与TMAdV具有非常高的相似性（93-99％），这表明松鼠猴可能是TMAdV的天然宿主.
