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Abstract：
In this work, we set out to provide an experimental setup, using Cs-131, with associated dosimetry for studying relative biological effectiveness (RBE) of Auger emitters.
Cs-131 decays by 100% electron capture producing K- (9%) and L- (80%) Auger electrons with mean energies of 26 keV and 3.5 keV, respectively, plus ≈ 9.4 very low energy electrons (<0.5 keV) per decay. Cs-131 accumulates in the cells through the Na+/K+-ATPase. By this uptake mechanism and the alkali chemistry of Cs+, we argue for its intracellular homogeneous distribution. Cs-131 was added to the cell culture medium of HeLa and V79 Cells. The bio-kinetics of Cs-131 (uptake, release, intracellular distribution) was examined by measuring its intracellular activity concentration over time. Taking advantage of the 100% confluent cellular monolayer, we developed a new and robust dosimetry that is entrusted to a quantity called SC-value.
The SC-values evaluated in the cell nucleus are almost independent of the nuclear size and geometry. We obtained dose-rate controlled RBE-values for intracellular Cs-131 decay. Using the γH2AX assay, the RBE was 1 for HeLa cells. Using the clonogenic cell survival, it was 3.9 for HeLa cells and 3.2 for V79 cells.
This experimental setup and dosimetry provides reliable RBE-values for Auger emitters in various cell lines.
Cs-131 decays by 100% electron capture producing K- (9%) and L- (80%) Auger electrons with mean energies of 26 keV and 3.5 keV, respectively, plus ≈ 9.4 very low energy electrons (<0.5 keV) per decay. Cs-131 accumulates in the cells through the Na+/K+-ATPase. By this uptake mechanism and the alkali chemistry of Cs+, we argue for its intracellular homogeneous distribution. Cs-131 was added to the cell culture medium of HeLa and V79 Cells. The bio-kinetics of Cs-131 (uptake, release, intracellular distribution) was examined by measuring its intracellular activity concentration over time. Taking advantage of the 100% confluent cellular monolayer, we developed a new and robust dosimetry that is entrusted to a quantity called SC-value.
The SC-values evaluated in the cell nucleus are almost independent of the nuclear size and geometry. We obtained dose-rate controlled RBE-values for intracellular Cs-131 decay. Using the γH2AX assay, the RBE was 1 for HeLa cells. Using the clonogenic cell survival, it was 3.9 for HeLa cells and 3.2 for V79 cells.
This experimental setup and dosimetry provides reliable RBE-values for Auger emitters in various cell lines.
摘要：
UNASSIGNED：在这项工作中，我们开始提供一个实验装置，使用Cs-131，与相关的剂量学研究俄歇发射器的相对生物有效性（RBE）。
未授权：Cs-131通过100％电子捕获衰减，产生平均能量为26keV和3.5keV的K-（9％）和L-（80％）俄歇电子，分别,每个衰变加上约9.4个非常低能量的电子（<0.5keV）。Cs-131通过Na+/K+-ATP酶在细胞中积累。通过这种吸收机制和Cs的碱性化学，我们认为它的细胞内均匀分布。将Cs-131加入HeLa和V79细胞的细胞培养基中。Cs-131的生物动力学(摄取，释放,细胞内分布）通过测量其随时间的细胞内活性浓度来检查。利用100%融合的细胞单层，我们开发了一种新的和强大的剂量测定，被委托给一个称为SC值的数量。
UNASSIGNED：在细胞核中评估的SC值几乎与细胞核的大小和几何形状无关。我们获得了细胞内Cs-131衰变的剂量率控制的RBE值。使用γH2AX测定法，HeLa细胞的RBE为1。利用克隆细胞的存活，HeLa细胞为3.9，V79细胞为3.2。
UNASSIGNED：此实验设置和剂量测定为各种细胞系中的俄歇发射器提供了可靠的RBE值。
未授权：Cs-131通过100％电子捕获衰减，产生平均能量为26keV和3.5keV的K-（9％）和L-（80％）俄歇电子，分别,每个衰变加上约9.4个非常低能量的电子（<0.5keV）。Cs-131通过Na+/K+-ATP酶在细胞中积累。通过这种吸收机制和Cs的碱性化学，我们认为它的细胞内均匀分布。将Cs-131加入HeLa和V79细胞的细胞培养基中。Cs-131的生物动力学(摄取，释放,细胞内分布）通过测量其随时间的细胞内活性浓度来检查。利用100%融合的细胞单层，我们开发了一种新的和强大的剂量测定，被委托给一个称为SC值的数量。
UNASSIGNED：在细胞核中评估的SC值几乎与细胞核的大小和几何形状无关。我们获得了细胞内Cs-131衰变的剂量率控制的RBE值。使用γH2AX测定法，HeLa细胞的RBE为1。利用克隆细胞的存活，HeLa细胞为3.9，V79细胞为3.2。
UNASSIGNED：此实验设置和剂量测定为各种细胞系中的俄歇发射器提供了可靠的RBE值。
