关键词: Axon Axon degeneration Coculture In vitro injury Neuro–glia interaction Schwann cell Spot culture

Mesh : Animals Axons / physiology Axotomy Cell Separation / methods Cells, Cultured Coculture Techniques / instrumentation methods Ganglia, Spinal / cytology embryology Immunomagnetic Separation / methods Laminin Mice Microscopy, Fluorescence / methods Nerve Growth Factor / pharmacology Polylysine Rats Rats, Sprague-Dawley Schwann Cells / cytology Sciatic Nerve / cytology Sensory Receptor Cells / cytology drug effects Wallerian Degeneration / physiopathology

来  源:   DOI:10.1007/978-1-0716-0585-1_8

Abstract:
Autonomous mechanisms of axon degeneration are frequently studied in vitro by mechanical axon injury of isolated sensory neurons. This has led to major advances in understanding the molecular pathways governing axon degeneration. However, this approach does not pay attention to potential glial mechanisms for the regulation of axon death. Here, I describe a straightforward protocol to seed purified rat Schwann cells on neuronal cultures in order to study the interaction between axons and these glia during axon degeneration.
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