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Abstract：
BACKGROUND: Biomaterials designed for tissue engineering should be nontoxic and nonimmunogenic and should achieve their intended functions. Treated dentin matrix (TDM), a bioactive extracellular matrix, is promising for tooth regeneration. However, the effect of sterilization on the surface properties of allogenous TDM in the animal model is unclear.
METHODS: The biological characteristics and influences of dental pulp stem cells (DPSCs) with autoclaved TDM (a-TDM) were studied using scanning electron microscopy, immunofluorescence microscopy, immunohistochemistry, and reverse transcription polymerase chain reaction in vitro. In addition, a-TDM was implanted in a mouse model for 6 weeks and was a substrate with DPSCs for tooth reconstruction in a goat animal model in vivo.
RESULTS: Allogenous a-TDM induced and supported DPSCs to develop new dentin pulp-like tissues, enamel dental pulp, and cementum periodontal complexes. Immunohistochemistry data showed that the markers dentin sialoprotein, βⅢ-tubulin, dentin matrix protein 1, amelogenin, VIII factors, type I collagen, cementum-derived attachment protein, and scleraxis transcription factor were positive stained in toothlike tissue.
CONCLUSIONS: Allogenous a-TDM served as an effective scaffold enabling DPSCs to proliferate and differentiate into a broad array of resident cells including odontoblasts, fibroblasts, vascular cells, and neural endings. Allogenous a-TDM with DPSCs can provide an ideal biomaterial for optimizing the regeneration of tooth material.
METHODS: The biological characteristics and influences of dental pulp stem cells (DPSCs) with autoclaved TDM (a-TDM) were studied using scanning electron microscopy, immunofluorescence microscopy, immunohistochemistry, and reverse transcription polymerase chain reaction in vitro. In addition, a-TDM was implanted in a mouse model for 6 weeks and was a substrate with DPSCs for tooth reconstruction in a goat animal model in vivo.
RESULTS: Allogenous a-TDM induced and supported DPSCs to develop new dentin pulp-like tissues, enamel dental pulp, and cementum periodontal complexes. Immunohistochemistry data showed that the markers dentin sialoprotein, βⅢ-tubulin, dentin matrix protein 1, amelogenin, VIII factors, type I collagen, cementum-derived attachment protein, and scleraxis transcription factor were positive stained in toothlike tissue.
CONCLUSIONS: Allogenous a-TDM served as an effective scaffold enabling DPSCs to proliferate and differentiate into a broad array of resident cells including odontoblasts, fibroblasts, vascular cells, and neural endings. Allogenous a-TDM with DPSCs can provide an ideal biomaterial for optimizing the regeneration of tooth material.
摘要：
背景：为组织工程设计的生物材料应无毒且无免疫原性，并应实现其预期功能。经处理的牙本质基质（TDM），生物活性细胞外基质，对牙齿再生很有希望。然而,在动物模型中，灭菌对同种异体TDM表面性质的影响尚不清楚。
方法：使用扫描电子显微镜研究了高压灭菌TDM（a-TDM）对牙髓干细胞（DPSCs）的生物学特性和影响，免疫荧光显微镜，免疫组织化学，和体外逆转录聚合酶链反应。此外,将a-TDM植入小鼠模型中持续6周,并且是具有DPSC的基质,用于在山羊动物模型中体内进行牙齿重建。
结果：同种异体a-TDM诱导并支持DPSC开发新的牙本质牙髓样组织，牙髓釉质，和牙骨质牙周复合物。免疫组织化学数据显示，标记牙本质唾液酸蛋白，βⅢ-微管蛋白，牙本质基质蛋白1，牙釉原蛋白，八因素，I型胶原蛋白，牙骨质来源的附着蛋白，牙样组织巩膜转录因子染色呈阳性。
结论：同种异体a-TDM作为一种有效的支架，使DPSC能够增殖和分化为包括成牙本质细胞在内的广泛的常驻细胞，成纤维细胞,血管细胞,和神经结局。具有DPSC的同种异体a-TDM可以为优化牙齿材料的再生提供理想的生物材料。
方法：使用扫描电子显微镜研究了高压灭菌TDM（a-TDM）对牙髓干细胞（DPSCs）的生物学特性和影响，免疫荧光显微镜，免疫组织化学，和体外逆转录聚合酶链反应。此外,将a-TDM植入小鼠模型中持续6周,并且是具有DPSC的基质,用于在山羊动物模型中体内进行牙齿重建。
结果：同种异体a-TDM诱导并支持DPSC开发新的牙本质牙髓样组织，牙髓釉质，和牙骨质牙周复合物。免疫组织化学数据显示，标记牙本质唾液酸蛋白，βⅢ-微管蛋白，牙本质基质蛋白1，牙釉原蛋白，八因素，I型胶原蛋白，牙骨质来源的附着蛋白，牙样组织巩膜转录因子染色呈阳性。
结论：同种异体a-TDM作为一种有效的支架，使DPSC能够增殖和分化为包括成牙本质细胞在内的广泛的常驻细胞，成纤维细胞,血管细胞,和神经结局。具有DPSC的同种异体a-TDM可以为优化牙齿材料的再生提供理想的生物材料。
