The Mechanism of the Stimforte Effect on the Activation of Target Cell Defense against Viral Infection.

• 发表时间: Mar 2020
• 来源期刊: Dokl Biol Sci
• DOI: 10.1134/S0012496620020076
• PMID: 32483708
• 文章类型: Journal Article
• 作者列表: Mal'dov DG,Deryabin PG,Grigoryan SS,Mishin DV,Andronova VL,Isaeva EI,Galegov GA,Ilichev AV

关键词: Stimforte; TLR-4 and NOD-2 receptors; Vero-V cells; hepatitis C virus; in vitro virus suppression; interferon production

Mesh: Acetylmuramyl-Alanyl-Isoglutamine / pharmacology; Adjuvants, Immunologic / administration & dosage pharmacology; Animals; Antiviral Agents / administration & dosage pharmacology; Cell Survival / drug effects; Chlorocebus aethiops; Dose-Response Relationship, Drug; Hepacivirus / drug effects physiology; Hepatitis C / drug therapy immunology; Interferons / metabolism; Lipopolysaccharides / administration & dosage pharmacology; Nod2 Signaling Adaptor Protein / metabolism; Organic Chemicals / administration & dosage pharmacology; Toll-Like Receptor 4 / metabolism; Vero Cells; Virus Replication / drug effects

Abstract

Stimforte in a wide range of concentrations (15-225 µg/mL) totally inhibits the cytopathic activity of hepatitis C virus (HCV) in the Vero-V cell culture. Interferons (IFN) play the most important role in the suppression of infection when the drug is introduced into the culture before the infection. When Stimforte is introduced after the infection, the mechanism of action seems to be different. The activators of IFN production are mainly (or exclusively) the ligands of receptor complexes TLR-4 and NOD-2 contained in the drug. The action of these substances is probably synergistic, similar to the action of LPS and MDP in Vero-V cells.