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Abstract：
BACKGROUND: Copper is an essential microelement for animals and has been used at pharmacological doses in weaned piglets to improve growth performance. However, it also induces systemic oxidative stress after short-term feeding. The aim of this study was to investigate the effects of dose and duration of dietary copper on lipid peroxidation and oxidative stress status in model of weaned piglets.
METHODS: A total of 48 crossbred piglets (weaned at 21d, weight ∼8.2 kg) were randomly assigned into 4 groups of 12 in each. The control group and 3 treatment groups fed with basal diet supplemented with 20, 100 and 200 mg/kg copper as copper sulfate for 3 and 6 weeks, respectively.
RESULTS: Dietary copper supplementation significantly affected the activities of ALP, LDH, LIPC and the levels of Ca and TG in serum as well as the copper and zinc deposition in liver. Increased MDA concentrations, and decreased GPX, CP and CAT concentrations in serum were found in 0, 100 and 200 mg Cu/kg diet groups at 3 weeks post weaning. Hepatic lipid peroxidation was also induced in these groups indicated from hepatic SOD1, GPX1, CAT, CP, MT1A and MT2A transcriptional levels. Those adverse symptoms were alleviative at 6 weeks post weaning. The hepatic Cu and Zn concentrations, serum MDA concentrations, and serum CAT and GPX activities were significantly correlated with Actinobacillus, Lactobacillus, Sarcina, Helicobacter, Campylobacterales, which could affect the intestinal health further.
CONCLUSIONS: These results indicated that copper deficiency or over supplementation would affect the systemic lipid peroxidation. These adverse changes were not observed when the dietary copper concentration at 20 mg Cu/kg diet. The results suggested the appropriate dietary copper concentration is around 20 mg Cu/kg diet, and its range might be much stricter than we thought.
METHODS: A total of 48 crossbred piglets (weaned at 21d, weight ∼8.2 kg) were randomly assigned into 4 groups of 12 in each. The control group and 3 treatment groups fed with basal diet supplemented with 20, 100 and 200 mg/kg copper as copper sulfate for 3 and 6 weeks, respectively.
RESULTS: Dietary copper supplementation significantly affected the activities of ALP, LDH, LIPC and the levels of Ca and TG in serum as well as the copper and zinc deposition in liver. Increased MDA concentrations, and decreased GPX, CP and CAT concentrations in serum were found in 0, 100 and 200 mg Cu/kg diet groups at 3 weeks post weaning. Hepatic lipid peroxidation was also induced in these groups indicated from hepatic SOD1, GPX1, CAT, CP, MT1A and MT2A transcriptional levels. Those adverse symptoms were alleviative at 6 weeks post weaning. The hepatic Cu and Zn concentrations, serum MDA concentrations, and serum CAT and GPX activities were significantly correlated with Actinobacillus, Lactobacillus, Sarcina, Helicobacter, Campylobacterales, which could affect the intestinal health further.
CONCLUSIONS: These results indicated that copper deficiency or over supplementation would affect the systemic lipid peroxidation. These adverse changes were not observed when the dietary copper concentration at 20 mg Cu/kg diet. The results suggested the appropriate dietary copper concentration is around 20 mg Cu/kg diet, and its range might be much stricter than we thought.
摘要：
背景：铜是动物必需的微量元素，已在断奶仔猪中以药理剂量使用，以改善生长性能。然而,它还会在短期喂养后引起全身氧化应激。本研究旨在研究日粮铜的剂量和持续时间对断奶仔猪脂质过氧化和氧化应激状态的影响。
方法：共48只杂交仔猪（断奶时间为21d，体重8.2公斤)随机分为4组，每组12人。对照组和3个治疗组饲喂基础日粮，添加20、100和200mg/kg硫酸铜，持续3周和6周，分别。
结果：膳食铜的添加显著影响ALP的活性，LDH,LIPC和血清中Ca和TG水平以及肝脏中铜和锌的沉积。MDA浓度增加，并减少GPX，断奶后3周，在0、100和200mgCu/kg饮食组中发现了血清中的CP和CAT浓度。在这些组中也诱导了肝脏脂质过氧化，这些表明来自肝脏SOD1，GPX1，CAT，CP,MT1A和MT2A转录水平。这些不良症状在断奶后6周缓解。肝Cu和Zn浓度，血清MDA浓度，血清CAT和GPX活性与放线杆菌显著相关，乳酸菌,Sarcina,螺杆菌,弯曲杆菌,这可能会进一步影响肠道健康。
结论：这些结果表明铜缺乏或过量补充会影响全身脂质过氧化。当膳食铜浓度为20mgCu/kg饮食时，未观察到这些不利变化。结果表明，适宜的日粮铜浓度为20mgCu/kg左右，它的范围可能比我们想象的要严格得多。
方法：共48只杂交仔猪（断奶时间为21d，体重8.2公斤)随机分为4组，每组12人。对照组和3个治疗组饲喂基础日粮，添加20、100和200mg/kg硫酸铜，持续3周和6周，分别。
结果：膳食铜的添加显著影响ALP的活性，LDH,LIPC和血清中Ca和TG水平以及肝脏中铜和锌的沉积。MDA浓度增加，并减少GPX，断奶后3周，在0、100和200mgCu/kg饮食组中发现了血清中的CP和CAT浓度。在这些组中也诱导了肝脏脂质过氧化，这些表明来自肝脏SOD1，GPX1，CAT，CP,MT1A和MT2A转录水平。这些不良症状在断奶后6周缓解。肝Cu和Zn浓度，血清MDA浓度，血清CAT和GPX活性与放线杆菌显著相关，乳酸菌,Sarcina,螺杆菌,弯曲杆菌,这可能会进一步影响肠道健康。
结论：这些结果表明铜缺乏或过量补充会影响全身脂质过氧化。当膳食铜浓度为20mgCu/kg饮食时，未观察到这些不利变化。结果表明，适宜的日粮铜浓度为20mgCu/kg左右，它的范围可能比我们想象的要严格得多。
