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Abstract：
The polydopamine coating on Au-Ag nanoparticles (Au-Ag-PDA) possess excellent photothermal conversion efficiency after absorbing near-infrared laser light. After the stem cell membrane (STCM) encapsulates Au-Ag-PDA (Au-Ag-PDA@STCM), the nanoparticles (NPs) exhibit less cytotoxicity, and further optimizing their efficiency in photothermal therapy. The photothermal activity of Au-Ag-PDA@STCM has not yet been reported. Therefore, in this study, the sebaceous gland cell line SZ95 and the golden hamsters were used to observe the photothermal effects of the Au-Ag-PDA@STCM. SZ95 cells were treated with various concen-trations of Au-Ag-PDA@STCM NPs. The photothermal effect on cell proliferation was analyzed after irradiating the cells with a 808 nm laser. After laser treatment of golden hamsters, the flank organs were observed at 4 different time points. Histological analysis was performed to observe tissue damage. The results suggest that Au-Ag-PDA@STCM NPs significantly inhibited the proliferation of sebaceous gland cells in vitro, and reduced the size of sebaceous glands and sebum secretion in vivo. Therefore, NPs can be used to treat acne by thermally injuring sebaceous gland cells.
摘要：
Au-Ag纳米颗粒（Au-Ag-PDA）上的聚多巴胺涂层在吸收近红外激光后具有出色的光热转换效率。在干细胞膜（STCM）封装Au-Ag-PDA（Au-Ag-PDA@STCM）后，纳米颗粒(NPs)表现出更小的细胞毒性，并进一步优化其在光热治疗中的效率。Au-Ag-PDA@STCM的光热活性还没有报导。因此,在这项研究中，用皮脂腺细胞系SZ95和金仓鼠观察Au-Ag-PDA@STCM的光热效应。用各种浓度的Au-Ag-PDA@STCMNP处理SZ95细胞。用808nm激光照射细胞后,分析对细胞增殖的光热效应。金黄地鼠激光治疗后，在4个不同的时间点观察侧腹器官。进行组织学分析以观察组织损伤。结果提示Au-Ag-PDA@STCMNPs能显著抑制皮脂腺细胞的增殖,并减少皮脂腺的大小和体内皮脂分泌。因此,NP可用于通过热损伤皮脂腺细胞来治疗痤疮。
