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Abstract：
Inflammation plays a negative role in the growth and development of bone. However, the underlining mechanisms of inflammation caused abnormal bone development and even bone disease are still poorly understood, especially in chickens. In this study, we explored the influence of inflammation on bone formation in broilers for the first time by using lipopolysaccharide (LPS) to establish systemic inflammatory models in chickens with tibia as the research object. The measurements of production and tibial parameters showed an inefficient production performance and lower growth rate in LPS group. We also found a large amount of platelets, inflammatory cells in chickens\' blood and higher levels of inflammatory factors in serum after LPS injection, meanwhile, increase in thrombus, chondrocyte nucleolysis, and osteoclasts and a reduction in blood vessels were observed in growth plate through histological observation. The qPCR analysis showed that the mRNA expression levels of NF-κB, TLR4, TF, TPO, and its receptor C-MPL enhanced, while VEGFA was inhibited in LPS group. In addition, in OPG/RANKL system, OPG was decreased while RANKL enhanced. It was also observed that the mRNA levels of MMP-9 and its inducing factor CD147 enhanced in LPS group. The western blot results were basically in consistent with mRNA test. Thus, we infer that inflammation can inhibit bone modeling and remodeling by affecting angiogenesis and osteogenesis, and result in negative effect on bone formation furtherly.
摘要：
炎症在骨骼的生长和发育中起负面作用。然而,引起骨发育异常甚至骨疾病的炎症机制仍然知之甚少，尤其是鸡。在这项研究中,以胫骨为研究对象，首次利用脂多糖（LPS）建立鸡全身炎症模型，探讨炎症对肉鸡骨形成的影响。生产和胫骨参数的测量显示，LPS组的生产性能低下，生长速率较低。我们还发现了大量的血小板,LPS注射后，鸡血液中的炎症细胞和血清中更高水平的炎症因子，同时,血栓增加，软骨细胞核溶解，通过组织学观察，在生长板中观察到破骨细胞和血管减少。qPCR分析表明NF-κB的mRNA表达水平,TLR4,TF,TPO,其受体C-MPL增强，LPS组VEGFA受到抑制。此外,在OPG/RANKL系统中，OPG降低而RANKL增强。还观察到LPS组MMP-9及其诱导因子CD147的mRNA水平增强。Westernblot结果与mRNA检测基本一致。因此，我们推断炎症可以通过影响血管生成和骨生成来抑制骨建模和重塑，并进一步对骨形成产生负面影响。
