关键词: AM fungi AM symbiosis Cell specificity Gene expression Laser microdissection

Mesh : Gene Expression Regulation, Fungal / genetics Laser Capture Microdissection / methods Medicago truncatula Mycorrhizae / genetics isolation & purification Oligonucleotide Array Sequence Analysis / methods Plant Roots / microbiology Symbiosis / genetics

来  源:   DOI:10.1007/978-1-0716-0603-2_13

Abstract:
Laser microdissection (LMD) technology has been widely applied to plant tissues, offering novel information on the role of different cell-type populations during plant-microbe interactions. In this chapter, protocols to apply the LMD approach to study plant and fungal transcript profiles in different cell-type populations from arbuscular mycorrhizal (AM) roots are described in detail, starting from the biological material preparation to gene expression analyses by RT-PCR and RT-qPCR.
摘要:
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