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Abstract：
OBJECTIVE: The aim of this work was to investigate three different mutations; Fec-B, FecXG, Fec-GH at three candidate genes; Bone morphogenetic protein receptor IB, Bone morphogenetic protein 15 and Growth Differentiation Factor 9, respectively, in six sheep breeds reared in Egypt namely; Rahmani, Barki, Rahmani X Barki cross, Awassi, Awassi X Suffolk cross, and Ossimi and their association with litter size.
RESULTS: Genomic DNA of 132 sheep was investigated for the Fec-B, FecXG, and Fec-GH mutations by Restriction Fragment Length Polymorphism, Single-Stranded Conformation Polymorphism and DNA sequencing. The results revealed that all breeds did not carry Fec-B mutation. On the other side, the mutations of FecXG, and Fec-GH were detected in Rahmani, and Rahmani X Barki cross which is associated with the high twinning rate/litter size of Rahmani (1.28) and Rahmani X Barki cross (1.22). While, the average litter size for other breeds had almost a constant values rate over six parities, ranging between 1.00 and 1.04.
RESULTS: Genomic DNA of 132 sheep was investigated for the Fec-B, FecXG, and Fec-GH mutations by Restriction Fragment Length Polymorphism, Single-Stranded Conformation Polymorphism and DNA sequencing. The results revealed that all breeds did not carry Fec-B mutation. On the other side, the mutations of FecXG, and Fec-GH were detected in Rahmani, and Rahmani X Barki cross which is associated with the high twinning rate/litter size of Rahmani (1.28) and Rahmani X Barki cross (1.22). While, the average litter size for other breeds had almost a constant values rate over six parities, ranging between 1.00 and 1.04.
摘要：
目的：这项工作的目的是研究三种不同的突变；Fec-B，FecXG,Fec-GH在三个候选基因；骨形态发生蛋白受体IB，骨形态发生蛋白15和生长分化因子9,在埃及饲养的六个绵羊品种中；Rahmani，Barki,RahmaniXBarki十字架,Awassi,AwassiX萨福克十字架，和Ossimi及其与产仔数的关系。
结果：对132只绵羊的基因组DNA进行了Fec-B研究，FecXG,和Fec-GH通过限制性片段长度多态性突变，单链构象多态性和DNA测序。结果表明，所有品种均未携带Fec-B突变。在另一边,FecXG的突变,在Rahmani中检测到Fec-GH，和RahmaniXBarki杂交，这与Rahmani（1.28）和RahmaniXBarki杂交（1.22）的高孪生率/产仔数有关。同时,其他品种的平均产仔数在六个对位上几乎是恒定的，范围在1.00和1.04之间。
结果：对132只绵羊的基因组DNA进行了Fec-B研究，FecXG,和Fec-GH通过限制性片段长度多态性突变，单链构象多态性和DNA测序。结果表明，所有品种均未携带Fec-B突变。在另一边,FecXG的突变,在Rahmani中检测到Fec-GH，和RahmaniXBarki杂交，这与Rahmani（1.28）和RahmaniXBarki杂交（1.22）的高孪生率/产仔数有关。同时,其他品种的平均产仔数在六个对位上几乎是恒定的，范围在1.00和1.04之间。
