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Abstract：
With the increased rate of stable remission after gonadotoxic cancer treatment, new methods of fertility preservation are required in order to provide the best possible care for oncological patients. Here, we report an original case of euploid blastocyst cryopreservation after in vitro maturation of ovarian tissue oocytes (OTO IVM). Thirty-three oocytes were obtained from the ovarian tissue after ovariectomy in the breast cancer patient. Six out of 12 matured oocytes fertilized successfully and 3 blastocysts were formed. Genetic investigation for mutations associated with this type of malignancy found that the patient is not a carrier. Preimplantation genetic testing was performed only for aneuploidies and found all 3 blastocysts to be euploid and suitable for embryo transfer. Our study showed that the ovarian tissue oocytes matured in vitro have the potential for euploid blastocyst formation after ICSI which could be screened for aneuploidies and inherited mutations and then be vitrified in order to provide the best fertility preservation strategy for women with cancer.
摘要：
随着性腺毒性肿瘤治疗后稳定缓解率的增加，为了为肿瘤患者提供最佳护理，需要新的生育能力保存方法。这里,我们报道了1例卵巢组织卵母细胞体外成熟（OTOIVM）后的整倍体囊胚冷冻保存的原始病例。乳腺癌患者卵巢切除术后，从卵巢组织中获得了33个卵母细胞。12个成熟卵母细胞中有6个成功受精，形成3个囊胚。对与这种恶性肿瘤相关的突变的遗传调查发现，患者不是携带者。仅对非整倍体进行了植入前遗传测试，发现所有3个胚泡都是整倍体，适合胚胎移植。我们的研究表明，体外成熟的卵巢组织卵母细胞在ICSI后具有形成整倍体囊胚的潜力，可以筛选非整倍体和遗传突变，然后玻璃化，以便为癌症女性提供最佳的生育力保护策略。
