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Abstract：
In soybean (Glycine max)-rhizobium interactions, the type III secretion system (T3SS) of rhizobium plays a key role in regulating host specificity. However, the lack of information on the role of T3SS in signaling networks limits our understanding of symbiosis. Here, we conducted an RNA sequencing analysis of three soybean chromosome segment substituted lines, one female parent and two derived lines with different chromosome-substituted segments of wild soybean and opposite nodulation patterns. By analyzing chromosome-linked differentially expressed genes in the substituted segments and quantitative trait loci (QTL)-assisted selection in the substituted-segment region, genes that may respond to type III effectors to mediate plant immunity-related signaling were identified. To narrow down the number of candidate genes, QTL assistant was used to identify the candidate region consistent with the substituted segments. Furthermore, one candidate gene, GmDRR1, was identified in the substituted segment. To investigate the role of GmDRR1 in symbiosis establishment, GmDRR1-overexpression and RNA interference soybean lines were constructed. The nodule number increased in the former compared with wild-type soybean. Additionally, the T3SS-regulated effectors appeared to interact with the GmDDR1 signaling pathway. This finding will allow the detection of T3SS-regulated effectors involved in legume-rhizobium interactions.
摘要：
在大豆(大豆)-根瘤菌相互作用中，根瘤菌的III型分泌系统（T3SS）在调节宿主特异性中起关键作用。然而,缺乏有关T3SS在信号网络中的作用的信息限制了我们对共生的理解.这里,我们对三个大豆染色体片段取代系进行了RNA测序分析，一个雌性亲本和两个衍生品系，它们具有不同的野生大豆染色体取代片段和相反的结瘤模式。通过分析取代片段中的染色体连锁差异表达基因和取代片段区域中的数量性状基因座（QTL）辅助选择，鉴定了可能响应III型效应子介导植物免疫相关信号的基因。为了缩小候选基因的数量，使用QTL助手来鉴定与取代片段一致的候选区域。此外，一个候选基因,GmDRR1在取代的片段中被鉴定。探讨GmDRR1在共生关系建立中的作用,构建了GmDRR1过表达和RNA干扰大豆品系。与野生型大豆相比，前者的根瘤数量增加。此外,T3SS调节的效应子似乎与GmDDR1信号通路相互作用.这一发现将允许检测豆科植物-根瘤菌相互作用中涉及的T3SS调节效应子。
