Abstract：
A new lanthanide (Ln3+)-dependent methanol-utilizing bacterial strain, La3113T, was isolated from rice field soil and its taxonomic position was investigated using polyphasic approaches. The strain was aerobic, Gram-stain-negative, strongly motile, catalase-positive and cytochrome oxidase-positive. It could neither catalyse the hydrolysis of urea nor reduce nitrate to nitrite. Growth was observed within a temperature range of 10-40 °C and a pH range of 6-8, with optimum growth at 28 °C and pH 7. Methylamine was utilized as the single source of energy, carbon and nitrogen, and it was oxidized by methylamine dehydrogenase. C16 : 1 ω7c, C16 : 1 ω6c and C16 : 0 were the dominant cellular fatty acids. Its draft genome (2.67 Mbp and 44.9 mol% G+C content) encodes genes including three Ln3+-dependent methanol dehydrogenase (XoxF-type MDH) genes, those for formaldehyde assimilation (ribulose monophosphate pathway), formate dehydrogenases and methylamine dehydrogenases, but not Ca2+-dependent MDH (MxaFI-MDH), which characterizes the species as a Ln3+-dependent methylotroph. The 16S rRNA gene sequence showed that strain La3113T belongs to the genus Methylotenera and is closely related to Methylotenera mobilis JLW8T (98.29 % identity). The digital DNA-DNA hybridization (dDDH) values (less than 30 %) and average nucleotide identity (ANI) values (less than 85 %) between genomes of strain La3113T and related type strains were lower than the thresholds for species delineation (70 % for dDDH and 95-96 % for ANI). On the basis of these polyphasic approaches, we propose a novel Methylotenera species, Methylotenera oryzisoli sp. nov. (type strain La3113T=NBRC 111954T=DSM 103219T).
摘要：
一种新的镧系元素（Ln3+）依赖的利用甲醇的细菌菌株，La3113T,从稻田土壤中分离出来，并使用多相方法研究了其分类位置。菌株是有氧的，革兰氏染色阴性，强烈的运动，过氧化氢酶阳性和细胞色素氧化酶阳性。它既不能催化尿素的水解，也不能将硝酸盐还原为亚硝酸盐。在10-40°C的温度范围和6-8的pH范围内观察到生长，在28°C和pH7下最佳生长。甲胺被用作单一能源，碳和氮，它被甲胺脱氢酶氧化。C16：1ω7c，C16:1ω6c和C16:0是主要的细胞脂肪酸。其基因组草案（2.67Mbp和44.9mol％GC含量）编码的基因包括三个Ln3依赖性甲醇脱氢酶（XoxF型MDH）基因，那些用于甲醛同化（单磷酸核酮糖途径），甲酸脱氢酶和甲胺脱氢酶，但不是Ca2+依赖的MDH（MxaFI-MDH），将该物种表征为Ln3依赖性甲基营养菌。16SrRNA基因序列表明，菌株La3113T属于甲硝胺属，与运动甲硝胺JLW8T密切相关(98.29%同一性)。La3113T菌株和相关类型菌株的基因组之间的数字DNA-DNA杂交（dDDH）值（小于30％）和平均核苷酸同一性（ANI）值（小于85％）低于物种划分的阈值（dDDH为70％，ANI为95-96％）。在这些多相方法的基础上，我们提出了一种新的甲虫物种，蛋黄碱。11月。(型应变La3113T=NBRC111954T=DSM103219T)。
