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Abstract：
The effects of different obturation techniques on calcium silicate-based sealer penetration in the presence of apically separated rotary files were evaluated. Forty-eight extracted mandibular incisors were used. ProTaper F2 rotary files were separated at the apical thirds. Samples were divided into four groups (n = 12) according to obturation technique used: (a) cold lateral compaction (CLC); (b) single cone; (c) bulk-fill (BF) without a core material; and (d) thermoplastic injection (TI). Specimens were sectioned horizontally at 1 and 3 mm from the apex and studied using a confocal scanning laser microscope. The maximum tubule penetration depth and percentage of penetration were measured. Data were statistically analyzed using parametric and nonparametric tests with a significance level of 5%. Regarding penetration depth, a significant difference was found at 1 mm (p < .05), while no significant difference was found at 3 mm (p > .05). At the 3 mm level, all of the obturation techniques showed similar penetration depths. Regarding penetration percentage, the values of the CLC and TI groups were statistically less when compared with the BF group at 1 and 3 mm levels, respectively (p < .05). Under the limitations of this in vitro study, results suggest that the obturation technique may present a significant effect on sealer penetration.
摘要：
评估了在顶部分离的旋转文件存在下，不同封堵技术对硅酸钙基密封剂渗透的影响。使用了48个下颌切牙。ProTaperF2旋转文件在顶端的三分之二处分开。根据所使用的闭塞技术将样品分为四组(n=12):(a)冷横向压实(CLC);(b)单锥体;(c)没有芯材料的整体填充(BF);和(d)热塑性注射(TI)。在距顶点1和3mm处水平切片样品,并使用共聚焦扫描激光显微镜进行研究。测量了最大细管穿透深度和穿透百分比。使用参数和非参数检验对数据进行统计分析，显著性水平为5％。关于穿透深度，在1mm处发现了显著差异(p<.05)，而在3mm处无显著差异(p>.05)。在3毫米的水平，所有的闭塞技术都显示出相似的穿透深度。关于渗透率，与1和3mm水平的BF组相比，CLC和TI组的值在统计学上较低，分别（p<0.05）。在这项体外研究的局限性下，结果表明，封堵技术可能对封堵剂的渗透有显著影响。
