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Abstract：
Background: Lung cancer is the most common malignant tumor worldwide. Accumulating results have shown that long non-coding RNAs (lncRNAs) play a key role in tumorigenesis. Patients and Methods: A total of 163 tumor tissues were collected from non-small cell lung cancer (NSCLC) patients from West China Hospital of Sichuan University. LincRNA00494 is a novel lncRNA, and its expression and biological effect in NSCLC were reported in this study. NSCLC cell lines were used in this study. Results: LincRNA00494 is mainly distributed in the cytoplasm. LincRNA00494 was downregulated in the tumor tissues compared with the adjacent non-tumor tissues. LincRNA00494 expression was positively correlated with SRCIN1 expression (R = 0.57, P < 0.05). Silencing of LincRNA00494 in the cell lines substantially decreased SRCIN1 expression at the mRNA and protein levels, whereas overexpression of LincRNA00494 enhanced the SRCIN1 levels. miR-150-3p significantly decreased the luciferase signals of LincRNA00494 and SRCIN1 reporters. After transfection with miR-150-3p mimics and miR-150-3p inhibitor, overexpression of LincRNA00494 decreased the proliferation of the H358 (36%) and H1299 (29%) cell lines compared with that of the control cells, as shown by CCK-8 assays, whereas silencing LincRNA00494 promoted the proliferation of the H358 (47%) and H1299 (35%) cells. Tumor growth from LincRNA00494-overexpressing xenografts was significantly decreased; additionally, LincRNA00494 silencing substantially increased tumor growth compared with that of the control cells. Conclusions: Functional experiments revealed that LincRNA00494 inhibited NSCLC cell proliferation, which might be related to the suppression of SRCIN1, a tumor suppressor gene, by acting as a decoy for miR-150-3p. The data showed that LincRNA00494 might have antineoplastic effects during NSCLC tumorigenesis through its role as a ceRNA.
摘要：
背景：肺癌是世界范围内最常见的恶性肿瘤。积累的结果表明，长链非编码RNA（lncRNA）在肿瘤发生中起关键作用。患者与方法:收集四川大学华西医院非小细胞肺癌(NSCLC)患者肿瘤组织163例。LincRNA00494是一种新型的lncRNA，本研究报道了其在NSCLC中的表达和生物学效应。在本研究中使用NSCLC细胞系。结果：LincRNA00494主要分布在细胞质中。与邻近的非肿瘤组织相比,LincRNA00494在肿瘤组织中下调。LincRNA00494的表达与SRCIN1的表达呈正相关(R=0.57,P<0.05)。沉默细胞系中的LincRNA00494在mRNA和蛋白质水平上显著降低了SRCIN1的表达,而LincRNA00494的过表达增强了SRCIN1水平。miR-150-3p显著降低了LincRNA00494和SRCIN1报告基因的荧光素酶信号。转染miR-150-3p模拟物和miR-150-3p抑制剂后，与对照细胞相比，LincRNA00494的过表达降低了H358（36％）和H1299（29％）细胞系的增殖，如CCK-8测定所示，而沉默LincRNA00494促进H358(47%)和H1299(35%)细胞的增殖。来自LincRNA00494过表达的异种移植物的肿瘤生长显着降低；此外，与对照细胞相比,LincRNA00494沉默显著增加了肿瘤生长。结论：功能实验显示，LincRNA00494抑制NSCLC细胞增殖，这可能与肿瘤抑制基因SRCIN1的抑制有关，通过充当miR-150-3p的诱饵。数据显示，LincRNA00494可能通过其作为ceRNA的作用在NSCLC肿瘤发生过程中具有抗肿瘤作用。
