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Abstract：
With ongoing colony losses driven in part by the Varroa mite and the associated exacerbation of the virus load, there is an urgent need to protect honey bees (Apis mellifera) from fatal levels of virus infection and from the non-target effects of insecticides used in agricultural settings. A continuously replicating cell line derived from the honey bee would provide a valuable tool for the study of molecular mechanisms of virus-host interaction, for the screening of antiviral agents for potential use within the hive, and for the assessment of the risk of current and candidate insecticides to the honey bee. However, the establishment of a continuously replicating honey bee cell line has proved challenging. Here, we provide an overview of attempts to establish primary and continuously replicating hymenopteran cell lines, methods (including recent results) of establishing honey bee cell lines, challenges associated with the presence of latent viruses (especially Deformed wing virus) in established cell lines and methods to establish virus-free cell lines. We also describe the potential use of honey bee cell lines in conjunction with infectious clones of honey bee viruses for examination of fundamental virology.
摘要：
由于部分由瓦螨螨和相关的病毒载量恶化导致的持续菌落损失，迫切需要保护蜜蜂（Apismellifera）免受致命的病毒感染和农业环境中使用的杀虫剂的非目标作用。来自蜜蜂的连续复制细胞系将为研究病毒与宿主相互作用的分子机制提供有价值的工具，用于筛选在蜂巢内潜在使用的抗病毒药物，并评估当前和候选杀虫剂对蜜蜂的风险。然而,事实证明，建立连续复制的蜜蜂细胞系具有挑战性。这里,我们概述了建立原代和连续复制膜翅目细胞系的尝试，建立蜜蜂细胞系的方法(包括最近的结果)，与在已建立的细胞系中存在潜伏病毒(尤其是变形的翼状病毒)相关的挑战和建立无病毒细胞系的方法。我们还描述了蜜蜂细胞系与蜜蜂病毒的感染性克隆一起用于基本病毒学检查的潜在用途。
